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验证数据展示

  • WB analysis using 12641-1-AP

    WB analysis using 12641-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 12641-1-AP

    IHC staining of rat brain using 12641-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12641-1-AP (SHC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 12641-1-AP

    IHC staining of mouse brain using 12641-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12641-1-AP (SHC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 12641-1-AP

    IHC staining of rat brain using 12641-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12641-1-AP (SHC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 12641-1-AP

    IF Staining of SH-SY5Y using 12641-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SHC4 antibody (12641-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 12641-1-AP

    IF Staining of HepG2 using 12641-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC4 antibody (12641-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

SHC4 Polyclonal antibody

SHC4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12641-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (2)

别名

SHC transforming protein 4, SH2 domain protein C4, RaLP, Rai-like protein, Rai like protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, human brain tissue, HepG2 cells, human skeletal muscle tissue, mouse brain tissue, A375 cells, rat brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, SH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12641-1-AP targets SHC4 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, CoIP, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 SHC4 fusion protein Ag3336 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SHC (Src homology 2 domain containing) family, member 4
别名 SHC transforming protein 4, SH2 domain protein C4, RaLP, Rai-like protein, Rai like protein
计算分子量 630 aa, 69 kDa
观测分子量 69 kDa
GenBank蛋白编号BC033907
基因名称 SHC4
Gene ID (NCBI) 399694
RRIDAB_10643243
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6S5L8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SHC4 antibody 12641-1-APDownload protocol
IHC protocol for SHC4 antibody 12641-1-APDownload protocol
IF protocol for SHC4 antibody 12641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Clin Sci (Lond)

Down-regulation of the insulin signaling pathway by SHC may correlate with congenital heart disease in Chinese populations.

Authors - Zhiling Luo
View Article
humanIHC,IF

PeerJ

A new 12-gene diagnostic biomarker signature of melanoma revealed by integrated microarray analysis.

Authors - Liu Wanting W
View Article
mouse,humanWB,IHC,IF,CoIP

Cancer Lett

SHC4 orchestrates β-catenin pathway-mediated metastasis in triple-negative breast cancer by promoting Src kinase autophosphorylation

Authors - Wenjing Zhong
  • KD Validated
View Article