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- KD/KO Validated
SHC Polyclonal antibody
SHC Polyclonal Antibody for IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HeLa cells, A431 cells, C6 cells, NIH/3T3 cells |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IF | See 1 publications below |
产品信息
12496-1-AP targets SHC in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, WB, ELISA |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | SHC fusion protein Ag3165 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | SHC (Src homology 2 domain containing) transforming protein 1 |
Synonyms | SH2 domain protein C1, SHC, SHC transforming protein 1, SHC transforming protein 3, SHC transforming protein A, SHC1, SHCA |
Calculated Molecular Weight | 66 kDa |
Observed Molecular Weight | 66 kDa, 52 kDa, 46 kDa |
GenBank Accession Number | BC014158 |
Gene Symbol | SHC1 |
Gene ID (NCBI) | 6464 |
RRID | AB_2187889 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P29353 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SHC antibody 12496-1-AP | Download protocol |
IF protocol for SHC antibody 12496-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Exp Cell Res LRTM1 promotes the differentiation of myoblast cells by negatively regulating the FGFR1 signaling pathway. | ||
Sci Adv HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis |