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验证数据展示

  • IHC staining of human stomach using 55373-1-AP (same clone as 55373-1-PBS)

    IHC staining of human stomach using 55373-1-AP (same clone as 55373-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55373-1-AP (SH3RF3 antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55373-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 55373-1-AP (same clone as 55373-1-PBS)

    IF Staining of U2OS using 55373-1-AP (same clone as 55373-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SH3RF3 antibody (55373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 55373-1-PBS in a different storage buffer formulation.

SH3RF3 Polyclonal antibody, PBS Only

SH3RF3 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 55373-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, Indirect ELISA

别名

POSH2, SH3MD4

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

55373-1-PBS targets SH3RF3 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3 domain containing ring finger 3
别名 POSH2, SH3MD4
计算分子量 93 kDa
GenBank蛋白编号NM_001099289
基因名称 SH3RF3
Gene ID (NCBI) 344558
RRIDAB_3743206
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TEJ3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SH3RF3 is a ubiquitously expressed RING finger E3 ligase that belongs to the POSH protein family. SH3RF3 also has a partial CRIB domain that has RAC1-binding activity(PMID: 20696164). SH3RF3 contains four SH3 domains for protein interactions and a ring finger domain that confers E3 ubiquitin ligase activity, possibly targeting itself and other proteins for proteasomal degradation (PMID: 41291187).

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