验证数据展示
经过测试的应用
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55373-1-AP targets SH3RF3 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SH3 domain containing ring finger 3 |
| 别名 | POSH2, SH3MD4 |
| 计算分子量 | 93 kDa |
| GenBank蛋白编号 | NM_001099289 |
| 基因名称 | SH3RF3 |
| Gene ID (NCBI) | 344558 |
| RRID | AB_3743206 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TEJ3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SH3RF3 is a ubiquitously expressed RING finger E3 ligase that belongs to the POSH protein family. SH3RF3 also has a partial CRIB domain that has RAC1-binding activity(PMID: 20696164). SH3RF3 contains four SH3 domains for protein interactions and a ring finger domain that confers E3 ubiquitin ligase activity, possibly targeting itself and other proteins for proteasomal degradation (PMID: 41291187).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SH3RF3 antibody 55373-1-AP | Download protocol |
| IHC protocol for SH3RF3 antibody 55373-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
