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验证数据展示

  • FC experiment of HeLa using CL488-67841

    FC experiment of HeLa using CL488-67841

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SH3BP5 (CL488-67841, Clone:1B9D4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated SH3BP5 Monoclonal antibody

SH3BP5 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67841

产品说明书

CloneNo. 1B9D4

宿主/亚型

Mouse / IgG1

种属反应性

Human, rabbit, mouse, rat, pig, chicken

应用

FC (Intra)

别名

SH3BP-5, SH3BP 5, SH3 domain-binding protein that preferentially associates with BTK, SH3 domain-binding protein 5, SH3 domain binding protein 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67841-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67841 targets SH3BP5 in FC (Intra) applications and shows reactivity with Human, rabbit, mouse, rat, pig, chicken samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, rabbit, mouse, rat, pig, chicken
免疫原 SH3BP5 fusion protein Ag30252 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SH3-domain binding protein 5 (BTK-associated)
别名 SH3BP-5, SH3BP 5, SH3 domain-binding protein that preferentially associates with BTK, SH3 domain-binding protein 5, SH3 domain binding protein 5
计算分子量 47 kDa
观测分子量75 kDa
GenBank蛋白编号BC010123
基因名称 SH3BP5
Gene ID (NCBI) 9467
RRIDAB_2934574
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60239
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH3BP5(SH3 domain-binding protein 5) is also named as SAB. It is previously isolated in a screen for proteins capable of binding to the Src homology 3 (SH3) domain of Bruton's tyrosine kinase (Btk) and could play a role in targeting JNK and Btk to mitochondria and/or mediating cross-talk between the JNK and Btk signalling pathways. It has 2 isoforms produced by alternative splicing with the molecular mass of 50 kDa and 32 kDa. There are two bands of 83 kDa and 70 kDa can be detected in A431 and the larger species may correspond to splice variants of SH3BP5 or SH3BP5 proteins that have undergone post-translational modification(PMID:12167088). In addition, we detect the band of 32 kDa as the isoform 2 of SH3BP5 and another band of 62 kDa besides the 47 kDa and 80 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SH3BP5 antibody CL488-67841Download protocol
Standard Protocols
Click here to view our Standard Protocols