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CoraLite® Plus 488-conjugated SH3BP4 Monoclonal antibody

SH3BP4 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2G4H3

Cat No : CL488-68045

Print datasheet

Synonyms

BOG25, EH binding protein 10, EHB10, SH3 domain binding protein 4, SH3BP4, TTP

验证数据展示

  • FC experiment of HeLa using CL488-68045

    FC experiment of HeLa using CL488-68045

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SH3BP4 (CL488-68045, Clone:2G4H3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68045-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68045 targets SH3BP4 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra)
Tested Reactivity Human
Immunogen SH3BP4 fusion protein Ag31238 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SH3-domain binding protein 4
Synonyms BOG25, EH binding protein 10, EHB10, SH3 domain binding protein 4, SH3BP4, TTP
Calculated Molecular Weight 963 aa, 108 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC057396
Gene Symbol SH3BP4
Gene ID (NCBI) 23677
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9P0V3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SH3BP4 antibody CL488-68045Download protocol
Standard Protocols
Click here to view our Standard Protocols