验证数据展示
经过测试的应用
| Positive WB detected in | Y79 cells, U2OS cells, A549 cells, HeLa cells, HepG2 cells, hTERT-RPE1 cells |
Planning an IHC experiment? We recommend our IHCeasy® SH3BP4 Ready-To-Use IHC Kit. SH3BP4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68045-1-Ig targets SH3BP4 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag31238 Product name: Recombinant human SH3BP4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-193 aa of BC057396 Sequence: MAAQRIRAANSNGLPRCKSEGTLIDLSEGFSETSFNDIKVPSPSALLVDNPTPFGNAKEVIAIKDYCPTNFTTLKFSKGDHLYVLDTSGGEWWYAHNTTEMGYIPSSYVQPLNYRNSTLSDSGMIDNLPDSPDEVAKELELLGGWTDDKKVPGRMYSNNPFWNGVQTNPFLNGNVPVMPSLDELNPKSTVDLL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | SH3-domain binding protein 4 |
| 别名 | BOG25, EH binding protein 10, EHB10, SH3 domain binding protein 4, SH3BP4, TTP |
| 计算分子量 | 963 aa, 108 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC057396 |
| 基因名称 | SH3BP4 |
| Gene ID (NCBI) | 23677 |
| RRID | AB_2918787 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9P0V3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SH3BP4 antibody 68045-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
