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  • Featured Product
  • KD/KO Validated

SH3BP4 Polyclonal antibody

SH3BP4 Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 17691-1-AP

Print datasheet

Synonyms

BOG25, EH binding protein 10, EHB10, SH3 domain binding protein 4, SH3BP4, TTP

验证数据展示

  • WB analysis using 17691-1-AP

    WB analysis using 17691-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17691-1-AP (SH3BP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 17691-1-AP

    IP experiment of mouse lung using 17691-1-AP

    IP result of anti-SH3BP4 (IP:17691-1-AP, 4ug; Detection:17691-1-AP 1:300) with mouse lung tissue lysate 3600ug.

  • IF Staining of HeLa using 17691-1-AP

    IF Staining of HeLa using 17691-1-AP

    Immunofluorescent analysis of HeLa cells using 17691-1-AP (SH3BP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17691-1-AP targets SH3BP4 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, pig
Immunogen SH3BP4 fusion protein Ag11847 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH3-domain binding protein 4
Synonyms BOG25, EH binding protein 10, EHB10, SH3 domain binding protein 4, SH3BP4, TTP
Calculated Molecular Weight 963 aa, 108 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC057396
Gene Symbol SH3BP4
Gene ID (NCBI) 23677
RRIDAB_2189364
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P0V3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SH3BP4 antibody 17691-1-APDownload protocol
IF protocol for SH3BP4 antibody 17691-1-APDownload protocol
IP protocol for SH3BP4 antibody 17691-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Exp Mol Med

SH3BP4, a novel pigmentation gene, is inversely regulated by miR-125b and MITF.

Authors - Kyu-Han Kim
  • KD Validated
View Article
pigWB

J Appl Physiol (1985)

Differential regulation of mTORC1 activation by leucine and β-hydroxy-β-methylbutyrate in skeletal muscle of neonatal pigs.

Authors - Agus Suryawan
View Article

Nat Commun

Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival

Authors - Joanne Watson
View Article