• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68328-1-Ig (same clone as 68328-1-PBS)

    WB analysis using 68328-1-Ig (same clone as 68328-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68328-1-Ig (SH3BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68328-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 68328-1-Ig (same clone as 68328-1-PBS)

    FC experiment of HepG2 using 68328-1-Ig (same clone as 68328-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug SH3BP1 Monoclonal antibody (68328-1-Ig, Clone:1B12C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68328-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68328-1-Ig (same clone as 68328-1-PBS)

    IF Staining of HeLa using 68328-1-Ig (same clone as 68328-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH3BP1 antibody (68328-1-Ig, Clone: 1B12C6 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68328-1-PBS in a different storage buffer formulation.

SH3BP1 Monoclonal antibody, PBS Only

SH3BP1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68328-1-PBS

产品说明书

CloneNo. 1B12C6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

SH3 domain-binding protein 1, SH3 domain binding protein 1, 3BP 1, 1B12C6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

68328-1-PBS targets SH3BP1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SH3BP1 fusion protein Ag15165 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SH3-domain binding protein 1
别名 SH3 domain-binding protein 1, SH3 domain binding protein 1, 3BP 1, 1B12C6
计算分子量 701 aa, 76 kDa
观测分子量76-80 kDa
GenBank蛋白编号BC008282
基因名称 SH3BP1
Gene ID (NCBI) 23616
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y3L3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SH3BP1 is also named as Chimeric SH3BP1-PDXP protein and BARGIN, and belongs to the to the RhoGAP family. SH3BP1 can be as a partner of the exocyst complex and establish a physical and functional link between two motility-driving pathways, the Ral/exocyst and Rac signaling pathways. (PMID:21658605). SH3BP1 localizes together with the exocyst to the leading edge of motile cells and that SH3BP1 regulates cell migration via its GAP activity upon Rac1.