• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of mouse heart using 21161-1-AP

    WB analysis of mouse heart using 21161-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 21161-1-AP (SH3BGR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 21161-1-AP

    IHC staining of human heart using 21161-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21161-1-AP (SH3BGR Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 21161-1-AP

    IHC staining of human heart using 21161-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21161-1-AP (SH3BGR Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 21161-1-AP

    IF Staining of HeLa using 21161-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH3BGR antibody (21161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

SH3BGR Polyclonal antibody

SH3BGR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21161-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

21 GARP, 21 glutamic acid rich protein, SH3BGR, SH3BGR protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21161-1-AP targets SH3BGR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human
免疫原 SH3BGR fusion protein Ag14739 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3 domain binding glutamic acid-rich protein
别名 21 GARP, 21 glutamic acid rich protein, SH3BGR, SH3BGR protein
计算分子量 239 aa, 26 kDa
观测分子量 26-30 kDa
GenBank蛋白编号BC006371
基因名称 SH3BGR
Gene ID (NCBI) 6450
RRIDAB_2878820
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55822
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SH3BGR antibody 21161-1-APDownload protocol
IHC protocol for SH3BGR antibody 21161-1-APDownload protocol
IF protocol for SH3BGR antibody 21161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Int J Mol Sci

SH3-Binding Glutamic Acid Rich-Deficiency Augments Apoptosis in Neonatal Rat Cardiomyocytes.

Authors - Anushka Deshpande
  • KD Validated
View Article