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验证数据展示

  • WB analysis of HEK-293 using 55320-1-AP

    WB analysis of HEK-293 using 55320-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55320-1-AP (SH2D5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 55320-1-AP

    IHC staining of human cerebellum using 55320-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cerebellum using 55320-1-AP

    IHC staining of human cerebellum using 55320-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55320-1-AP (SH2D5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 55320-1-AP

    IF Staining of HEK-293 using 55320-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SH2D5 antibody (55320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SH2D5 Polyclonal antibody

SH2D5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 55320-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

SH2 domain-containing protein 5, SH2 domain containing 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55320-1-AP targets SH2D5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH2 domain containing 5
别名 SH2 domain-containing protein 5, SH2 domain containing 5
计算分子量 37 kDa
观测分子量 43-47 kDa
GenBank蛋白编号NM_001103160
基因名称 SH2D5
Gene ID (NCBI) 400745
RRIDAB_2881307
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZV89
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH2D5, or Src homology 2 domain-containing protein 5, is a mammalian-specific adaptor-like protein that contains an N-terminal phosphotyrosine-binding domain and a C-terminal Src homology 2 (SH2) domain. SH2D5 has been found to interact with the breakpoint cluster region protein, a RacGAP that is also highly expressed in the brain. This interaction occurs between the phosphotyrosine-binding domain of SH2D5 and an NxxF motif located within the N-terminal region of the breakpoint cluster region. Furthermore, SH2D5 has been implicated in the regulation of cell growth in hepatocellular carcinoma cells, where it binds to transketolase (TKT) and promotes the activity of STAT3, a key transcription factor involved in cell proliferation and survival.

实验方案

Product Specific Protocols
WB protocol for SH2D5 antibody 55320-1-APDownload protocol
IHC protocol for SH2D5 antibody 55320-1-APDownload protocol
IF protocol for SH2D5 antibody 55320-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols