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验证数据展示

  • IF Staining of HEK-293 using CL488-55320

    IF Staining of HEK-293 using CL488-55320

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 SH2D5 antibody (CL488-55320) at dilution of 1:200.

  • IF Staining of HEK-293 using CL488-55320

    IF Staining of HEK-293 using CL488-55320

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 SH2D5 antibody (CL488-55320) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated SH2D5 Polyclonal antibody

SH2D5 Polyclonal Antibody for IF/ICC
Cat No. CL488-55320

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

SH2 domain-containing protein 5, SH2 domain containing 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 55320-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-55320 targets SH2D5 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH2 domain containing 5
别名 SH2 domain-containing protein 5, SH2 domain containing 5
计算分子量 37 kDa
观测分子量 43-47 kDa
GenBank蛋白编号NM_001103160
基因名称 SH2D5
Gene ID (NCBI) 400745
RRIDAB_3672858
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen Affinity Purified
UNIPROT IDQ6ZV89
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH2D5, or Src homology 2 domain-containing protein 5, is a mammalian-specific adaptor-like protein that contains an N-terminal phosphotyrosine-binding domain and a C-terminal Src homology 2 (SH2) domain. SH2D5 has been found to interact with the breakpoint cluster region protein, a RacGAP that is also highly expressed in the brain. This interaction occurs between the phosphotyrosine-binding domain of SH2D5 and an NxxF motif located within the N-terminal region of the breakpoint cluster region. Furthermore, SH2D5 has been implicated in the regulation of cell growth in hepatocellular carcinoma cells, where it binds to transketolase (TKT) and promotes the activity of STAT3, a key transcription factor involved in cell proliferation and survival.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SH2D5 antibody CL488-55320Download protocol
Standard Protocols
Click here to view our Standard Protocols