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验证数据展示

  • IHC staining of human ovary cancer using 12226-1-AP (same clone as 12226-1-PBS)

    IHC staining of human ovary cancer using 12226-1-AP (same clone as 12226-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12226-1-AP (SH2B1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12226-1-AP (same clone as 12226-1-PBS)

    IF Staining of HeLa using 12226-1-AP (same clone as 12226-1-PBS)

    Immunofluorescent analysis of HeLa cells using 12226-1-AP (SH2B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

  • WB analysis using 12226-1-AP (same clone as 12226-1-PBS)

    WB analysis using 12226-1-AP (same clone as 12226-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12226-1-AP (SH2B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 12226-1-AP (same clone as 12226-1-PBS)

    IP experiment of HepG2 using 12226-1-AP (same clone as 12226-1-PBS)

    IP result of anti-SH2B1 (IP:12226-1-AP, 4ug; Detection:12226-1-AP 1:1500) with HepG2 cells lysate 1080 ug. This data was developed using the same antibody clone with 12226-1-PBS in a different storage buffer formulation.

SH2B1 Polyclonal antibody, PBS Only

SH2B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12226-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

KIAA1299, Pro-rich, PH and SH2 domain-containing signaling mediator, PSM, SH2 B, SH2 domain-containing protein 1B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12226-1-PBS targets SH2B1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2863

Product name: Recombinant human SH2B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC010704

Sequence: MVQREELLSFMGAEEAAPDPAGVGRGGGVAGPPSGGGGQPQWQKCRLLLRSEGEGGGGSRLEFFVPPKASRPRLSIPCSSITDVRTTTALEMPDRENTFVVKVEGPSEYIMETVDAQHVKAWVSDIQECLSPGPCPATSPRPMTLPLAPGTSFLTRENTDSLELSCLNHSESLPSQDLLLGPSESNDRLSQGAYGGLSDRPSASISPSSASIAASHFDSMELLPPELPPRIPIEEGPPAGTVHPLSAPYPPLDTPETATGSFLFQGEPEGGEGDQPLSGYPWFHGMLSRLKAAQLALTGGTGSHGVFLVRQSETRRGEYVLTFNFQGKAKHLRLSLNEEGQCRVQHLWFQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH2B adaptor protein 1
别名 KIAA1299, Pro-rich, PH and SH2 domain-containing signaling mediator, PSM, SH2 B, SH2 domain-containing protein 1B
计算分子量 426 aa, 45 kDa
观测分子量 70-80 kDa
GenBank蛋白编号BC010704
基因名称 SH2B1
Gene ID (NCBI) 25970
RRIDAB_2877838
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRF2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SH2B1 is a member of the SH2-domain containing mediators family. SH2B1 has been shown to interact with INS receptor, Grb2, TrkA and Janus kinase 2. Variations close to or in the SH2B1 gene have been found to associate with obesity in two large genome wide association studies of body mass index (PMID: 19079260). SH2B1deletions are associated with severe early-onset obesity (PMID: 19966786).

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