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验证数据展示

  • WB analysis using 20825-1-AP (same clone as 20825-1-PBS)

    WB analysis using 20825-1-AP (same clone as 20825-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20825-1-AP (SGSM3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 20825-1-AP (same clone as 20825-1-PBS)

    IHC staining of human heart using 20825-1-AP (same clone as 20825-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20825-1-AP (SGSM3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SGSM3 antibody included
  • IHC staining of human heart using 20825-1-AP (same clone as 20825-1-PBS)

    IHC staining of human heart using 20825-1-AP (same clone as 20825-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20825-1-AP (SGSM3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SGSM3 antibody included
  • IF Staining of HEK-293 using 20825-1-AP (same clone as 20825-1-PBS)

    IF Staining of HEK-293 using 20825-1-AP (same clone as 20825-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SGSM3 antibody (20825-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 20825-1-AP (same clone as 20825-1-PBS)

    IP experiment of mouse brain using 20825-1-AP (same clone as 20825-1-PBS)

    IP result of anti-SGSM3 (IP:20825-1-AP, 4ug; Detection:20825-1-AP 1:2000) with mouse brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 20825-1-AP (same clone as 20825-1-PBS)

    IF Staining of HeLa using 20825-1-AP (same clone as 20825-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SGSM3 antibody (20825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20825-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SGSM3 Polyclonal antibody, PBS Only

SGSM3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20825-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

MAP, Merlin associated protein, Merlin-associated protein, RabGAPLP, Rab-GTPase-activating protein-like protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SGSM3 Ready-To-Use IHC Kit. SGSM3 primary antibody included.

产品信息

20825-1-PBS targets SGSM3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14800

Product name: Recombinant human SGSM3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 400-749 aa of BC008078

Sequence: RTQRRKSTITALLFGEDDLEALKAKNIKQTELVADLREAILRVARHFQCTDPKNCSVELTPDYSMESHQRDHENYVACSRSHRRRAKALLDFERHDDDELGFRKNDIITIVSQKDEHCWVGELNGLRGWFPAKFVEVLDERSKEYSIAGDDSVTEGVTDLVRGTLCPALKALFEHGLKKPSLLGGACHPWLFIEEAAGREVERDFASVYSRLVLCKTFRLDEDGKVLTPEELLYRAVQSVNVTHDAVHAQMDVKLRSLICVGLNEQVLHLWLEVLCSSLPTVEKWYQPWSFLRSPGWVQIKCELRVLCCFAFSLSQDWELPAKREAQQPLKEGVRDMLVKHHLFSWDVDG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 small G protein signaling modulator 3
别名 MAP, Merlin associated protein, Merlin-associated protein, RabGAPLP, Rab-GTPase-activating protein-like protein
计算分子量 749 aa, 85 kDa
观测分子量 85 kDa
GenBank蛋白编号BC008078
基因名称 SGSM3
Gene ID (NCBI) 27352
RRIDAB_10693680
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96HU1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Small G protein signaling modulator 3 (SGSM3) is also named as MAP, RABGAPLP and RABGAPLP. SGSM3 is highly correlated with Cx43 in heart functions and that high levels of SGSM3 may induce Cx43 turnover through lysosomal degradation in infarcted rat hearts (PMID: 29577900, PMID: 32271805). SGSM3 has been shown to be associated with small G-protein-coupled receptor signaling (PMID: 29693742). Several studies have shown that SGSM3 is associated with the risk of some cancers, including liver, breast, colorectal and bladder cancers (PMID: 32271805).

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