SGSM3 Polyclonal antibody
SGSM3 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, L02 cells, mouse brain tissue, mouse testis tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human heart tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
The immunogen of 20825-1-AP is SGSM3 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Immunogen | SGSM3 fusion protein Ag14800 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | small G protein signaling modulator 3 |
| Synonyms | MAP, Merlin associated protein, RabGAPLP, RUSC3, RUTBC3, SGSM3 |
| Calculated Molecular Weight | 749 aa, 85 kDa |
| Observed Molecular Weight | 85 kDa |
| GenBank Accession Number | BC008078 |
| Gene Symbol | SGSM3 |
| Gene ID (NCBI) | 27352 |
| RRID | AB_10693680 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96HU1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SGSM3 antibody 20825-1-AP | Download protocol |
| IHC protocol for SGSM3 antibody 20825-1-AP | Download protocol |
| IF protocol for SGSM3 antibody 20825-1-AP | Download protocol |
| IP protocol for SGSM3 antibody 20825-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep AP-1/σ1A and AP-1/σ1B adaptor-proteins differentially regulate neuronal early endosome maturation via the Rab5/Vps34-pathway. | ||
Mediators Inflamm Autophagy Contributes to Host Immunity and Protection against Zika Virus Infection via Type I IFN Signaling. | ||
Cell Rep Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease. | ||
J Cell Biol TBC1D18 is a Rab5-GAP that coordinates endosome maturation together with Mon1
|
