验证数据展示
经过测试的应用
Positive WB detected in | mouse skeletal muscle tissue |
Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11936-1-AP targets Beta Sarcoglycan in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | Beta Sarcoglycan fusion protein Ag2533 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | sarcoglycan, beta (43kDa dystrophin-associated glycoprotein) |
别名 | 43DAG, A3b, Beta sarcoglycan, Beta SG, LGMD2E, SGC, SGCB |
计算分子量 | 318 aa, 35 kDa |
观测分子量 | 45-48 kDa |
GenBank蛋白编号 | BC020709 |
基因名称 | Beta Sarcoglycan |
Gene ID (NCBI) | 6443 |
RRID | AB_10699870 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q16585 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SGCB is a 43-kD component of the dystrophin-glycoprotein complex.
实验方案
Product Specific Protocols | |
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WB protocol for Beta Sarcoglycan antibody 11936-1-AP | Download protocol |
IHC protocol for Beta Sarcoglycan antibody 11936-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
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