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Beta Sarcoglycan Polyclonal antibody

Beta Sarcoglycan Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11936-1-AP

Print datasheet

Synonyms

43DAG, A3b, Beta sarcoglycan, Beta SG, LGMD2E, SGC, SGCB

验证数据展示

  • WB analysis of mouse skeletal muscle using 11936-1-AP

    WB analysis of mouse skeletal muscle using 11936-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11936-1-AP (Beta Sarcoglycan antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 11936-1-AP

    IHC staining of human colon using 11936-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11936-1-AP (Beta Sarcoglycan antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SGCB antibody included

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SGCB Ready-To-Use IHC Kit. SGCB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11936-1-AP targets Beta Sarcoglycan in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen Beta Sarcoglycan fusion protein Ag2533 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sarcoglycan, beta (43kDa dystrophin-associated glycoprotein)
Synonyms 43DAG, A3b, Beta sarcoglycan, Beta SG, LGMD2E, SGC, SGCB
Calculated Molecular Weight 318 aa, 35 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC020709
Gene Symbol SGCB
Gene ID (NCBI) 6443
RRIDAB_10699870
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16585
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SGCB is a 43-kD component of the dystrophin-glycoprotein complex.

实验方案

Product Specific Protocols
WB protocol for Beta Sarcoglycan antibody 11936-1-APDownload protocol
IHC protocol for Beta Sarcoglycan antibody 11936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Phytomedicine

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Authors - Liping Zhang
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mouseWB,IF

Oncotarget

Liraglutide suppresses obesity and induces brown fat-like phenotype via Soluble Guanylyl Cyclase mediated pathway in vivo and in vitro.

Authors - Endong Zhu
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