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Host
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0
Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 87600-1-RR (same clone as 87600-1-PBS)

    WB analysis using 87600-1-RR (same clone as 87600-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87600-1-RR (SF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87600-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 87600-1-RR (same clone as 87600-1-PBS)

    IF Staining of HepG2 using 87600-1-RR (same clone as 87600-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SF4 antibody (87600-1-RR, Clone: 252925A4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87600-1-PBS in a different storage buffer formulation.

  • Affinity of 87600-1-RR

    Affinity of 87600-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87600-1-RR against Human SF4 were performed. The affinity constant is below 1 pM.

SF4 Recombinant monoclonal antibody, PBS Only

SF4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87600-1-PBS

产品说明书

CloneNo. 252925A4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

F23858, RNA-binding protein RBP, Splicing factor 4, SUGP1, SURP and G-patch domain-containing protein 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87600-1-PBS targets SF4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8006

Product name: Recombinant human SF4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-348 aa of BC002986

Sequence: LYEPNSQGYKYYRQKLEEFRKAKASSTGSFTAPDPGLKRKSPPEALSGSLPPATTCPASSTPAPTIIPAPAAPGKPASAATVKRKRKSRWGPEEDKVELPPAELVQRDVDASPSPLSVQDLKGLGYEKGKPVGLVGVTELSDAQKKQLKEQQEMQQMYDMIMQHKRAMQDMQLLWEKAVQQHQHGYDSDEEVDSELGTWEHQLRRMEMDKTREWAEQLTKMGRGKHFIGDFLPPDELEKFMETFKALKEGREPDYSEYKEFKLTVENIGYQMLMKMGWKEGEGLGSEGQGIKNPVNKGTTTVDGAGFGIDRPAELSKEDDEYEAFRKRMMLAYRFRPNPLNNPRRPYY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 splicing factor 4
别名 F23858, RNA-binding protein RBP, Splicing factor 4, SUGP1, SURP and G-patch domain-containing protein 1
计算分子量 72 kDa
观测分子量72-75 kDa
GenBank蛋白编号BC002986
基因名称 SF4
Gene ID (NCBI) 57794
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8IWZ8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SF4 (Splicing factor 4), also known as SUGP1 (SURP and G-patch domain-containing protein 1) was first identified as a putative splicing factor based on domain composition analysis. SUGP1 protein is predicted to contain two SURP motifs, domains of alternative splicing regulators thought to be involved in RNA binding, as well as a G-patch domain, a region of six highly conserved glycine residues commonly found in eukaryotic RNA-processing proteins (PMID: 27206982). Research has shown that SUGP1 is involved in the regulation of cholesterol metabolism (PMID: 31474574).

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