验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, rat kidney tissue, HEK-293 cells, K-562 cells, HL-60 cells, LNCaP cells, Neuro-2a cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86170-2-RR targets SF3A1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 |
CatNo: Ag8196 Product name: Recombinant human SF3A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 443-793 aa of BC007684 Sequence: DRSIREKQSDDEVYAPGLDIESSLKQLAERRTDIFGVEETAIGKKIGEEEIQKPEEKVTWDGHSGSMARTQQAAQANITLQEQIEAIHKAKGLVPEDDTKEKIGPSKPNEIPQQPPPPSSATNIPSSAPPITSVPRPPTMPPPVRTTVVSAVPVMPRPPMASVVRLPPGSVIAPMPPIIHAPRINVVPMPPSAPPIMAPRPPPMIVPTAFVPAPPVAPVPAPAPMPPVHPPPPMEDEPTSKKLKTEDSLMPEEEFLRRNKGPVSIKVQVPNMQDKTEWKLNGQVLVFTLPLTDQVSVIKVKIHEATGMPAGKQKLQYEGIFIKDSNSLAYYNMANGAVIHLALKERGGRKK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | splicing factor 3a, subunit 1, 120kDa |
| 别名 | PRP21, SAP 114, SAP114, SF3a120, Spliceosome-associated protein 114 |
| 计算分子量 | 793 aa, 89 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC007684 |
| 基因名称 | SF3A1 |
| Gene ID (NCBI) | 10291 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15459 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SF3A1 antibody 86170-2-RR | Download protocol |
| WB protocol for SF3A1 antibody 86170-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
