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验证数据展示

  • WB analysis using 86170-2-RR

    WB analysis using 86170-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86170-2-RR (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat kidney using 86170-2-RR

    WB analysis of rat kidney using 86170-2-RR

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 86170-2-RR (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 86170-2-RR

    IF Staining of HeLa using 86170-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SF3A1 antibody (86170-2-RR, Clone: 250768F4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SF3A1 Recombinant antibody

SF3A1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86170-2-RR

产品说明书

CloneNo. 250768F4

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

别名

PRP21, SAP 114, SAP114, SF3a120, Spliceosome-associated protein 114

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, rat kidney tissue, HEK-293 cells, K-562 cells, HL-60 cells, LNCaP cells, Neuro-2a cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SF3A1 Ready-To-Use IHC Kit. SF3A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86170-2-RR targets SF3A1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 SF3A1 fusion protein Ag8196 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 splicing factor 3a, subunit 1, 120kDa
别名 PRP21, SAP 114, SAP114, SF3a120, Spliceosome-associated protein 114
计算分子量 793 aa, 89 kDa
观测分子量120 kDa
GenBank蛋白编号BC007684
基因名称 SF3A1
Gene ID (NCBI) 10291
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15459
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )

实验方案

Product Specific Protocols
WB protocol for SF3A1 antibody 86170-2-RRDownload protocol
IF protocol for SF3A1 antibody 86170-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols