验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, K-562 cells, HL-60 cells, LNCaP cells, Neuro-2a cells |
Planning an IHC experiment? We recommend our IHCeasy SF3A1 Ready-To-Use IHC Kit. SF3A1 primary antibody included.
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
86170-1-RR targets SF3A1 in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | SF3A1 fusion protein Ag8196 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | splicing factor 3a, subunit 1, 120kDa |
别名 | PRP21, SAP 114, SAP114, SF3a120, Spliceosome-associated protein 114 |
计算分子量 | 793 aa, 89 kDa |
观测分子量 | 120 kDa |
GenBank蛋白编号 | BC007684 |
基因名称 | SF3A1 |
Gene ID (NCBI) | 10291 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q15459 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )
实验方案
Product Specific Protocols | |
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WB protocol for SF3A1 antibody 86170-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |