验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, C6 cells, mouse brain tissue, mouse kidney tissue, L02 cells, rat brain tissue, NIH/3T3 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24840-1-AP targets SETD7 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag18219 Product name: Recombinant human SETD7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-366 aa of BC121055 Sequence: MDSDDEMVEEAVEGHLDDDGLPHGFCTVTYSSTDRFEGNFVHGEKNGRGKFFFFDGSTLEGYYVDDALQGQGVYTYEDGGVLQGTYVDGELNGPAQEYDTDGRLIFKGQYKDNIRHGVCWIYYPDGGSLVGEVNEDGEMTGEKIAYVYPDERTALYGKFIDGEMIEGKLATLMSTEEGRPHFELMPGNSVYHFDKSTSSCISTNALLPDPYESERVYVAESLISSAGEGLFSKVAVGPNTVMSFYNGVRITHQEVDSRDWALNGNTLSLDEETVIDVPEPYNHVSKYCASLGHKANHSFTPNCIYDMFVHPRFGPIKCIRTLRAVEADEELTVAYGYDHSPPGKSGPEAPEWYQVELKAFQATQQK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SET domain containing (lysine methyltransferase) 7 |
| 别名 | EC:2.1.1.364, H3 K4 HMTase SETD7, H3-K4-HMTase SETD7, Histone H3-K4 methyltransferase SETD7, Histone-lysine N-methyltransferase SETD7 |
| 计算分子量 | 366 aa, 41 kDa |
| 观测分子量 | 48-50 kDa |
| GenBank蛋白编号 | BC121055 |
| 基因名称 | SETD7 |
| Gene ID (NCBI) | 80854 |
| RRID | AB_2737406 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WTS6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SET domain containing lysine methyltransferase 7 (SETD7, also called SET7/9) was the first lysine methyltransferase (KMT) discovered to specifically monomethylate lysine-4 of histone 3 (H3K4me1) (PMID: 26435321). SETD7 has been shown to have a very broad target specificity in vitro, including transcriptional regulators such as TAF10, p53, ER, p65, STAT3, Rb, Mypt, Tat, and Foxo3 (PMID: 25136132, 30013796).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SETD7 antibody 24840-1-AP | Download protocol |
| WB protocol for SETD7 antibody 24840-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Bioact Mater Dynamic hydrogels orchestrate the differentiation fate of mesenchymal stem cells through epigenetic regulation of SETD7 to accelerate bone defect repair. | ||
Cell Death Dis ACAT2 suppresses the ubiquitination of YAP1 to enhance the proliferation and metastasis ability of gastric cancer via the upregulation of SETD7
| ||
Mol Ther Integrated stress response promotes acute liver failure by activating SETD7 and enhancing NLRP3 methylation. | ||
EBioMedicine Histone-lysine N-methyltransferase SETD7 is a potential serum biomarker for colorectal cancer patients. | ||
Acta Pharmacol Sin Inhibition of Setd7 protects against cardiomyocyte hypertrophy via inhibiting lipid oxidation.
| ||
Biol Direct SETD7-mediated epigenetic regulation of LncRNA XIST promotes trophoblast pyroptosis: a study in preeclampsia mouse and cell models. |
