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SETD1A Monoclonal antibody
SETD1A Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1C2G12
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, LNCaP cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
67936-1-Ig targets SETD1A in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | SETD1A fusion protein Ag30424 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | SET domain containing 1A |
| Synonyms | hSET1A, KIAA0339, KMT2F, Lysine N methyltransferase 2F, SET domain containing 1A, Set1, Set1A, SETD1A |
| Calculated Molecular Weight | 186 kDa |
| Observed Molecular Weight | 270 kDa |
| GenBank Accession Number | NM_014712 |
| Gene Symbol | SETD1A |
| Gene ID (NCBI) | 9739 |
| RRID | AB_2918688 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O15047 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SETD1A is a SET domain-containing methyltransferase involved in the epigenetic regulation of transcription(PMID:12670868). It is the main catalytic component of a multiprotein complex that methylates lysine 4 of histone H3, a histone mark associated with gene activation (PMID:29937342). Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis (PMID:31197650). Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated (PubMed:12670868). SETD1A has a calculated molecular weight of 186 kDa, and a 258 kDa band was also reported (PMID: 31076518).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SETD1A antibody 67936-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration | ||
Signal Transduct Target Ther CXXC finger protein 1 (CFP1) bridges the reshaping of genomic H3K4me3 signature to the advancement of lung adenocarcinoma |
