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SETD1A Monoclonal antibody

SETD1A Monoclonal Antibody for WB, ELISA
Cat No. 67936-1-Ig

产品说明书

CloneNo. 1C2G12

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat and More (1)

应用

WB, ELISA and More (1)

hSET1A, KIAA0339, KMT2F, Lysine N methyltransferase 2F, SET domain containing 1A, Set1, Set1A, SETD1A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, LNCaP cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67936-1-Ig targets SETD1A in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IF
经测试反应性 Human, mouse, rat
文献引用反应性human, mouse
免疫原 SETD1A fusion protein Ag30424 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SET domain containing 1A
别名 hSET1A, KIAA0339, KMT2F, Lysine N methyltransferase 2F, SET domain containing 1A, Set1, Set1A, SETD1A
计算分子量 186 kDa
观测分子量270 kDa
GenBank蛋白编号NM_014712
基因名称 SETD1A
Gene ID (NCBI) 9739
RRIDAB_2918688
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO15047
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SETD1A is a SET domain-containing methyltransferase involved in the epigenetic regulation of transcription(PMID:12670868). It is the main catalytic component of a multiprotein complex that methylates lysine 4 of histone H3, a histone mark associated with gene activation (PMID:29937342). Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis (PMID:31197650). Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated (PubMed:12670868). SETD1A has a calculated molecular weight of 186 kDa, and a 258 kDa band was also reported (PMID: 31076518).

实验方案

Product Specific Protocols
WB protocol for SETD1A antibody 67936-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang
human,mouseWB

Nat Commun

Early-life exercise induces immunometabolic epigenetic modification enhancing anti-inflammatory immunity in middle-aged male mice

Authors - Nini Zhang
humanIF

J Inflamm (Lond)

Histone H3 posttranslational modified enzymes defined neutrophil plasticity and their vulnerability to IL-10 in the course of the inflammation

Authors - Paweł Piatek
humanWB

Cancer Lett

PARP1 interacts with WDR5 to enhance target gene recognition and facilitate tumorigenesis

Authors - Yali Qin
humanWB

Signal Transduct Target Ther

CXXC finger protein 1 (CFP1) bridges the reshaping of genomic H3K4me3 signature to the advancement of lung adenocarcinoma

Authors - Tao Fan
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