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验证数据展示

  • WB analysis using 84823-3-RR

    WB analysis using 84823-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84823-3-RR (SESN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 84823-3-RR

    IF Staining of HepG2 using 84823-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SESN3 antibody (84823-3-RR, Clone: 242189D1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of mouse liver using 84823-3-RR

    IHC staining of mouse liver using 84823-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84823-3-RR (SESN3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84823-3-RR

    Affinity and Kinetic Characterization of 84823-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84823-3-RR against Human SESN3 were performed. The affinity constant is 6.78 nM.

SESN3 Recombinant antibody

SESN3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84823-3-RR

产品说明书

CloneNo. 242189D1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Sestrin-3, sestrin 3, SEST3, EC:1.11.1.-, 242189D1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, A431 cells, HepG2 cells, HEK-293 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84823-3-RR targets SESN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SESN3 fusion protein Ag17872 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sestrin 3
别名 Sestrin-3, sestrin 3, SEST3, EC:1.11.1.-, 242189D1
计算分子量 492 aa, 57 kDa
观测分子量57-60 kDa
GenBank蛋白编号BC017296
基因名称 SESN3
Gene ID (NCBI) 143686
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP58005
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT-induced increase in ROS. It might be a promising target in selectively killing cancer cells with high AKT activity levels.

实验方案

Product Specific Protocols
WB protocol for SESN3 antibody 84823-3-RRDownload protocol
IHC protocol for SESN3 antibody 84823-3-RRDownload protocol
IF protocol for SESN3 antibody 84823-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols