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验证数据展示

  • WB analysis using 84823-3-RR (same clone as 84823-3-PBS)

    WB analysis using 84823-3-RR (same clone as 84823-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84823-3-RR (SESN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84823-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 84823-3-RR (same clone as 84823-3-PBS)

    IHC staining of mouse liver using 84823-3-RR (same clone as 84823-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84823-3-RR (SESN3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84823-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84823-3-RR (same clone as 84823-3-PBS)

    IF Staining of HepG2 using 84823-3-RR (same clone as 84823-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SESN3 antibody (84823-3-RR, Clone: 242189D1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84823-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84823-3-RR

    Affinity and Kinetic Characterization of 84823-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84823-3-RR against Human SESN3 were performed. The affinity constant is 6.78 nM.

SESN3 Recombinant monoclonal antibody, PBS Only

SESN3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84823-3-PBS

产品说明书

CloneNo. 242189D1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

242189D1, EC:1.11.1.-, SEST3, sestrin 3, Sestrin-3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84823-3-PBS targets SESN3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag17872

Product name: Recombinant human SESN3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-321 aa of BC017296

Sequence: MNRGGGSPSAAANYLLCTNCRKVLRKDKRIRVSQPLTRGPSAFIPEKEVVQANTVDERTNFLVEEYSTSGRLDNITQVMSLHTQYLESFLRSQFYMLRMDGPLPLPYRHYIAIMAAARHQCSYLINMHVDEFLKTGGIAEWLNGLEYVPQRLKNLNEINKLLAHRPWLITKEHIQKLVKTGENNWSLPELVHAVVLLAHYHALASFVFGSGINPERDPEISNGFRLISVNNFCVCDLANDNNIENASLSGSNFGIVDSLSELEALMERMKRLQEEREDEEASQEEMSTRFEKEKKESLFVVSGDTFHSFPHSGAFLHFFAF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sestrin 3
别名 242189D1, EC:1.11.1.-, SEST3, sestrin 3, Sestrin-3
计算分子量 492 aa, 57 kDa
观测分子量57-60 kDa
GenBank蛋白编号BC017296
基因名称 SESN3
Gene ID (NCBI) 143686
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP58005
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT-induced increase in ROS. It might be a promising target in selectively killing cancer cells with high AKT activity levels.

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