验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HuH-7 cells, L02 cells, U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy SERPINE1 Ready-To-Use IHC Kit. SERPINE1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85683-6-RR targets SERPINE1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | SERPINE1 fusion protein Eg3798 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 |
| 别名 | PAI-1, SERPINE1, PAI-1, PAI, PAI 1, PAI1 |
| 计算分子量 | 402 aa, 45 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC010860 |
| 基因名称 | PAI-1 |
| Gene ID (NCBI) | 5054 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P05121 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SERPINE1 antibody 85683-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |