验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse liver tissue, rat liver tissue, rat kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21170-1-AP targets SERPINB7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag15394 Product name: Recombinant human SERPINB7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 32-305 aa of BC069417 Sequence: SLSLFAALALVRLGAQDDSLSQIDKLLHVNTASGYGNSSNSQSGLQSQLKRVFSDINASHKDYDLSIVNGLFAEKVYGFHKDYIECAEKLYDAKVERVDFTNHLEDTRRNINKWVENETHGKIKNVIGEGGISSSAVMVLVNAVYFKGKWQSAFTKSETINCHFKSPKCSGKAVAMMHQERKFNLSVIEDPSMKILELRYNGGINMYVLLPENDLSEIENKLTFQNLMEWTNPRRMTSKYVEVFFPQFKIEKNYEMKQYLRALGLKDIFDESKA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade B (ovalbumin), member 7 |
| 别名 | Serpin B7, MEGSIN, TP55 |
| 计算分子量 | 380 aa, 43 kDa |
| 观测分子量 | 43-45 kDa |
| GenBank蛋白编号 | BC069417 |
| 基因名称 | SERPINB7 |
| Gene ID (NCBI) | 8710 |
| RRID | AB_3742020 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75635 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Serine protease inhibitor B7 (SERPINB7) is also named Serpin B7, Megsin and TP55. SerpinB7 is identified as a skin-specific endogenous protease inhibitor. SerpinB7 is upregulated in diabetic nephropathy and induces progressive mesangial expansion (PMID: 11877466). SERPINB7 is abundantly expressed in keratinocytes, with particularly high levels in the stratum corneum and stratum granulosum of the epidermis (PMID: 41469525). SERPINB7 promotes keratinocyte differentiation and dampens inflammation in psoriasis in a calcium-dependent manner (PMID: 35864103). SERPINB7 is expressed mostly in renal mesangial cells, and its expression is significantly increased in renal tissues of IgA and diabetic nephropathies. SERPINB7 regulates cell proliferation, signal transduction, and extracellular matrix metabolism (PMID: 37809412).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SERPINB7 antibody 21170-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
