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验证数据展示

  • WB analysis using 67633-1-Ig

    WB analysis using 67633-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67633-1-Ig (Maspin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67633-1-Ig

    WB analysis of HeLa using 67633-1-Ig

    WB result of Maspin antibody (67633-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Maspin transfected HeLa cells.

  • IHC staining of human oesophagus cancer using 67633-1-Ig

    IHC staining of human oesophagus cancer using 67633-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67633-1-Ig (Maspin antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human oesophagus cancer using 67633-1-Ig

    IHC staining of human oesophagus cancer using 67633-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67633-1-Ig (Maspin antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 67633-1-Ig

    IF Staining of HEK-293 using 67633-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Maspin antibody (67633-1-Ig, Clone: 3G5A3 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Maspin Monoclonal antibody

Maspin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67633-1-Ig

产品说明书

CloneNo. 3G5A3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

SERPINB5, 3G5A3, Peptidase inhibitor 5, PI 5, PI5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, HUMAN saliva, A431 cells
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67633-1-Ig targets Maspin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19129

Product name: Recombinant human SERPINB5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-231 aa of BC020713

Sequence: MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFPESETKECPFRVNKVCGAACSSKRSPIIDVKNDRDRVGHKSIPMRNLRARPAKCLS

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade B (ovalbumin), member 5
别名 SERPINB5, 3G5A3, Peptidase inhibitor 5, PI 5, PI5
计算分子量 375 aa, 42 kDa
观测分子量 38-40 kDa
GenBank蛋白编号BC020713
基因名称 Maspin
Gene ID (NCBI) 5268
RRIDAB_2882834
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP36952
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINB5, also named PI-5, Maspin, and Serpin B5, belongs to the serpin family and Ov-serpin subfamily. It is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, SERPINB5 exhibits no serine protease inhibitory activity.

实验方案

Product Specific Protocols
IF protocol for Maspin antibody 67633-1-IgDownload protocol
IHC protocol for Maspin antibody 67633-1-IgDownload protocol
WB protocol for Maspin antibody 67633-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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