验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, Human saliva, HeLa cells, A431 cells |
Positive IHC detected in | human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:800-1:3200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67633-1-Ig targets Maspin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | Human |
免疫原 | Maspin fusion protein Ag19129 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | serpin peptidase inhibitor, clade B (ovalbumin), member 5 |
别名 | maspin, Peptidase inhibitor 5, PI 5, PI5, Serpin B5, SERPINB5, SPB5 |
计算分子量 | 375 aa, 42 kDa |
观测分子量 | 38-40 kDa |
GenBank蛋白编号 | BC020713 |
基因名称 | Maspin |
Gene ID (NCBI) | 5268 |
RRID | AB_2882834 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P36952 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SERPINB5, also named as PI-5, Maspin and Serpin B5, belongs to the serpin family and Ov-serpin subfamily. It is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, SERPINB5 exhibits no serine protease inhibitory activity.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Maspin antibody 67633-1-Ig | Download protocol |
IHC protocol for Maspin antibody 67633-1-Ig | Download protocol |
IF protocol for Maspin antibody 67633-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |