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Maspin Monoclonal antibody

Maspin Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

3G5A3

Cat No : 67633-1-Ig

Print datasheet

Synonyms

maspin, Peptidase inhibitor 5, PI 5, PI5, Serpin B5, SERPINB5, SPB5

验证数据展示

  • WB analysis using 67633-1-Ig

    WB analysis using 67633-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67633-1-Ig (Maspin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human oesophagus cancer using 67633-1-Ig

    IHC staining of human oesophagus cancer using 67633-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67633-1-Ig (Maspin antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human oesophagus cancer using 67633-1-Ig

    IHC staining of human oesophagus cancer using 67633-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 67633-1-Ig (Maspin antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, Human saliva, HeLa cells, A431 cells
Positive IHC detected inhuman oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67633-1-Ig targets Maspin in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity Human
Immunogen Maspin fusion protein Ag19129 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 5
Synonyms maspin, Peptidase inhibitor 5, PI 5, PI5, Serpin B5, SERPINB5, SPB5
Calculated Molecular Weight 375 aa, 42 kDa
Observed Molecular Weight 38-40 kDa
GenBank Accession NumberBC020713
Gene Symbol SERPINB5
Gene ID (NCBI) 5268
RRIDAB_2882834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36952
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINB5, also named as PI-5, Maspin and Serpin B5, belongs to the serpin family and Ov-serpin subfamily. It is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, SERPINB5 exhibits no serine protease inhibitory activity.

实验方案

Product Specific Protocols
WB protocol for Maspin antibody 67633-1-IgDownload protocol
IHC protocol for Maspin antibody 67633-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols