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  • Featured Product
  • KD/KO Validated

SERF2 Polyclonal antibody

SERF2 Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11691-1-AP

Print datasheet

Synonyms

4F5REL, FAM2C, H4F5rel, HsT17089, Protein 4F5 related, SERF2, small EDRK rich factor 2

验证数据展示

  • WB analysis of HepG2 using 11691-1-AP

    WB analysis of HepG2 using 11691-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11691-1-AP (SERF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 11691-1-AP

    IP experiment of HepG2 using 11691-1-AP

    IP result of anti-SERF2 (IP:11691-1-AP, 4ug; Detection:11691-1-AP 1:500) with HepG2 cells lysate 4000ug.

  • IF Staining of HepG2 using 11691-1-AP

    IF Staining of HepG2 using 11691-1-AP

    Immunofluorescent analysis of HepG2 cells using 11691-1-AP (SERF2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 11691-1-AP

    IF Staining of MCF-7 using 11691-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SERF2 antibody (11691-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IF detected inHepG2 cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11691-1-AP targets SERF2 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen SERF2 fusion protein Ag2305 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name small EDRK-rich factor 2
Synonyms 4F5REL, FAM2C, H4F5rel, HsT17089, Protein 4F5 related, SERF2, small EDRK rich factor 2
Calculated Molecular Weight 59 aa, 7 kDa
GenBank Accession NumberBC022326
Gene Symbol SERF2
Gene ID (NCBI) 10169
RRIDAB_10596937
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP84101
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERF2 Belongs to the SERF family. The antibody is specific to SERF2.

实验方案

Product Specific Protocols
WB protocol for SERF2 antibody 11691-1-APDownload protocol
IF protocol for SERF2 antibody 11691-1-APDownload protocol
IP protocol for SERF2 antibody 11691-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mamm Genome

A novel knockout mouse for the small EDRK-rich factor 2 (Serf2) showing developmental and other deficits.

Authors - Karen Cleverley
  • KD Validated
View Article
mouseWB

Life Sci Alliance

Deletion of SERF2 in mice delays embryonic development and alters amyloid deposit structure in the brain

Authors - Esther Stroo
View Article