验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11691-1-AP targets SERF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2305 Product name: Recombinant human SERF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-59 aa of BC022326 Sequence: MTRGNQRELARQKNMKKQSDSVKGKRRDDGLSAAARKQRDSEIMQQKQKKANEKKEEPK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | small EDRK-rich factor 2 |
| 别名 | small EDRK rich factor 2, Protein 4F5-related, Protein 4F5 related, h4F5rel, Gastric cancer-related protein VRG107 |
| 计算分子量 | 59 aa, 7 kDa |
| GenBank蛋白编号 | BC022326 |
| 基因名称 | SERF2 |
| Gene ID (NCBI) | 10169 |
| RRID | AB_10596937 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P84101 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SERF2 Belongs to the SERF family. The antibody is specific to SERF2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SERF2 antibody 11691-1-AP | Download protocol |
| IHC protocol for SERF2 antibody 11691-1-AP | Download protocol |
| IF protocol for SERF2 antibody 11691-1-AP | Download protocol |
| IP protocol for SERF2 antibody 11691-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mamm Genome A novel knockout mouse for the small EDRK-rich factor 2 (Serf2) showing developmental and other deficits.
| ||
Life Sci Alliance Deletion of SERF2 in mice delays embryonic development and alters amyloid deposit structure in the brain |