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SEPT9 Recombinant antibody

SEPT9 Recombinant Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IP, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E7

Cat No : 82844-2-RR

Print datasheet

Synonyms

AF17q25, FLJ75490, KIAA0991, MLL septin like fusion protein, MSF, MSF1, NAPB, Ov/Br septin, Ovarian/Breast septin, PNUTL4, SEPT9, SeptD1, septin 9, Septin D1, SINT1

验证数据展示

  • WB analysis using 82844-2-RR

    WB analysis using 82844-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 82844-2-RR

    WB analysis using 82844-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 82844-2-RR

    IP experiment of mouse brain using 82844-2-RR

    IP result of anti-SEPT9 (IP:82844-2-RR, 4ug; Detection:82844-2-RR 1:1000) with mouse brain tissue lysate 1680 ug.

经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, mouse brain tissue, mouse spleen tissue
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82844-2-RR targets SEPT9 in WB, IP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IP, WB, ELISA
Tested Reactivity Human, mouse
Immunogen SEPT9 fusion protein Ag1218 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name septin 9
Synonyms AF17q25, FLJ75490, KIAA0991, MLL septin like fusion protein, MSF, MSF1, NAPB, Ov/Br septin, Ovarian/Breast septin, PNUTL4, SEPT9, SeptD1, septin 9, Septin D1, SINT1
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 65 kDa, 38-40 kDa
GenBank Accession NumberBC021192
Gene Symbol SEPT9
Gene ID (NCBI) 10801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UHD8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).

实验方案

Product Specific Protocols
WB protocol for SEPT9 antibody 82844-2-RRDownload protocol
IP protocol for SEPT9 antibody 82844-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols