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验证数据展示

  • WB analysis using 82844-2-RR

    WB analysis using 82844-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 82844-2-RR

    WB analysis of A549 using 82844-2-RR

    WB result of SEPT9 antibody (82844-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEPT9 transfected A549 cells.

  • WB analysis of HepG2 using 82844-2-RR

    WB analysis of HepG2 using 82844-2-RR

    WB result of Septin 9 antibody (82844-2-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 9 transfected HepG2 cells.

  • WB analysis using 82844-2-RR

    WB analysis using 82844-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 82844-2-RR

    IP experiment of mouse brain using 82844-2-RR

    IP result of anti-SEPT9 (IP:82844-2-RR, 4ug; Detection:82844-2-RR 1:1000) with mouse brain tissue lysate 1680 ug.

  • IHC staining of human colon using 82844-2-RR

    IHC staining of human colon using 82844-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82844-2-RR (SEPT9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEPTIN9 antibody included
  • IHC staining of human colon cancer using 82844-2-RR

    IHC staining of human colon cancer using 82844-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82844-2-RR (SEPT9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SEPTIN9 antibody included
  • Affinity and Kinetic Characterization of 82844-2-RR

    Affinity and Kinetic Characterization of 82844-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82844-2-RR against Human SEPT9 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

Septin 9 Recombinant monoclonal antibody

Septin 9 Uni-rAb® Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 82844-2-RR

产品说明书

CloneNo. 2E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA

别名

SEPT9, 2E7, KIAA0991, MLL septin-like fusion protein, MLL septin-like fusion protein MSF-A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, mouse brain tissue, mouse spleen tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon tissue, human colon cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SEPTIN9 Ready-To-Use IHC Kit. SEPTIN9 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82844-2-RR targets Septin 9 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1218

Product name: Recombinant human SEPT9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 250-568 aa of BC021192

Sequence: RNEKAPVDFGYVGIDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVHFKQRITADLLSNGIDVYPQKEFDEDSEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSSIHFEAYRVKRLNEGSSAMANGVEEKEPEAPEM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 septin 9
别名 SEPT9, 2E7, KIAA0991, MLL septin-like fusion protein, MLL septin-like fusion protein MSF-A
计算分子量 64 kDa
观测分子量 65-80 kDa, 38-40 kDa
GenBank蛋白编号BC021192
基因名称 SEPT9
Gene ID (NCBI) 10801
RRIDAB_3670573
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UHD8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).

实验方案

Product Specific Protocols
IHC protocol for Septin 9 antibody 82844-2-RRDownload protocol
IP protocol for Septin 9 antibody 82844-2-RRDownload protocol
WB protocol for Septin 9 antibody 82844-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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