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验证数据展示

  • WB analysis using 82844-2-RR (same clone as 82844-2-PBS)

    WB analysis using 82844-2-RR (same clone as 82844-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 82844-2-RR (same clone as 82844-2-PBS)

    WB analysis of A549 using 82844-2-RR (same clone as 82844-2-PBS)

    WB result of SEPT9 antibody (82844-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEPT9 transfected A549 cells. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 82844-2-RR (same clone as 82844-2-PBS)

    WB analysis of HepG2 using 82844-2-RR (same clone as 82844-2-PBS)

    WB result of Septin 9 antibody (82844-2-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 9 transfected HepG2 cells. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

  • WB analysis using 82844-2-RR (same clone as 82844-2-PBS)

    WB analysis using 82844-2-RR (same clone as 82844-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82844-2-RR (SEPT9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 82844-2-RR (same clone as 82844-2-PBS)

    IP experiment of mouse brain using 82844-2-RR (same clone as 82844-2-PBS)

    IP result of anti-SEPT9 (IP:82844-2-RR, 4ug; Detection:82844-2-RR 1:1000) with mouse brain tissue lysate 1680 ug. This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 82844-2-RR (same clone as 82844-2-PBS)

    IHC staining of human colon using 82844-2-RR (same clone as 82844-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82844-2-RR (SEPT9 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SEPTIN9 antibody included
  • IHC staining of human colon cancer using 82844-2-RR (same clone as 82844-2-PBS)

    IHC staining of human colon cancer using 82844-2-RR (same clone as 82844-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82844-2-RR (SEPT9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82844-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SEPTIN9 antibody included
  • Affinity and Kinetic Characterization of 82844-2-RR

    Affinity and Kinetic Characterization of 82844-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82844-2-RR against Human SEPT9 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

Septin 9 Recombinant monoclonal antibody, PBS Only

Septin 9 Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 82844-2-PBS

产品说明书

CloneNo. 2E7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

SEPT9, 2E7, KIAA0991, MLL septin-like fusion protein, MLL septin-like fusion protein MSF-A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SEPTIN9 Ready-To-Use IHC Kit. SEPTIN9 primary antibody included.

产品信息

82844-2-PBS targets Septin 9 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1218

Product name: Recombinant human SEPT9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 250-568 aa of BC021192

Sequence: RNEKAPVDFGYVGIDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVHFKQRITADLLSNGIDVYPQKEFDEDSEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSSIHFEAYRVKRLNEGSSAMANGVEEKEPEAPEM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 septin 9
别名 SEPT9, 2E7, KIAA0991, MLL septin-like fusion protein, MLL septin-like fusion protein MSF-A
计算分子量 64 kDa
观测分子量 65-80 kDa, 38-40 kDa
GenBank蛋白编号BC021192
基因名称 SEPT9
Gene ID (NCBI) 10801
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UHD8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).

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