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SEPHS2 Polyclonal antibody

SEPHS2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14109-1-AP

Print datasheet

Synonyms

Selenide, water dikinase 2, Selenium donor protein 2, Selenophosphate synthase 2, selenophosphate synthetase 2, SEPHS2, SPS2

验证数据展示

  • WB analysis using 14109-1-AP

    WB analysis using 14109-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14109-1-AP (SEPHS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 14109-1-AP

    IHC staining of human breast cancer using 14109-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 14109-1-AP

    WB analysis using 14109-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14109-1-AP (SEPHS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNCI-H1299 cells, mouse pancreas tissue, mouse adipose tissue, rat liver tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

14109-1-AP targets SEPHS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SEPHS2 fusion protein Ag5255 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name selenophosphate synthetase 2
Synonyms Selenide, water dikinase 2, Selenium donor protein 2, Selenophosphate synthase 2, selenophosphate synthetase 2, SEPHS2, SPS2
Calculated Molecular Weight 47 kDa
Observed Molecular Weight47-50 kDa
GenBank Accession NumberBC016643
Gene Symbol SEPHS2
Gene ID (NCBI) 22928
RRIDAB_2878014
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99611
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SEPHS2 antibody 14109-1-APDownload protocol
IHC protocol for SEPHS2 antibody 14109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Med (Lausanne)

A glycometabolic gene signature associating with immune infiltration and chemosensitivity and predicting the prognosis of patients with osteosarcoma

Authors - Fengyan Wang
View Article