验证数据展示
经过测试的应用
| Positive FC detected in | PHA treated human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in a 100 µl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
APC-98324-3 targets SEMA7A in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg2005 Product name: Recombinant Human SEMA7A protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 45-648 aa of NM_003612.5 Sequence: QGHLRSGPRIFAVWKGHVGQDRVDFGQTEPHTVLFHEPGSSSVWVGGRGKVYLFDFPEGKNASVRTVNIGSTKGSCLDKRDCENYITLLERRSEGLLACGTNARHPSCWNLVNGTVVPLGEMRGYAPFSPDENSLVLFEGDEVYSTIRKQEYNGKIPRFRRIRGESELYTSDTVMQNPQFIKATIVHQDQAYDDKIYYFFREDNPDKNPEAPLNVSRVAQLCRGDQGGESSLSVSKWNTFLKAMLVCSDAATNKNFNRLQDVFLLPDPSGQWRDTRVYGVFSNPWNYSAVCVYSLGDIDKVFRTSSLKGYHSSLPNPRPGKCLPDQQPIPTETFQVADRHPEVAQRVEPMGPLKTPLFHSKYHYQKVAVHRMQASHGETFHVLYLTTDRGTIHKVVEPGEQEHSFAFNIMEIQPFRRAAAIQTMSLDAERRKLYVSSQWEVSQVPLDLCEVYGGGCHGCLMSRDPYCGWDQGRCISIYSSERSVLQSINPAEPHKECPNPKPDKAPLQKVSLAPNSRYYLSCPMESRHATYSWRHKENVEQSCEPGHQSPNCILFIENLTAQQYGHYFCEAQEGSYFREAQHWQLLPEDGIMAEHLLGHACALA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group) |
| 别名 | CD108, CDw108, John-Milton-Hargen human blood group Ag, Sema K1, Sema L |
| 计算分子量 | 75kDa |
| GenBank蛋白编号 | NM_003612.5 |
| 基因名称 | SEMA7A |
| Gene ID (NCBI) | 8482 |
| 偶联类型 | APC Fluorescent Dye |
| 最大激发/发射波长 | 650 nm / 660 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O75326-1 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
SEMA7A, also named CD108 and SEMAL, belongs to the semaphorin family. SEMA7A is the only membrane-associated glycosylphosphatidylinositol (GPI)-linked semaphorin and plays a role in the regulation of neuronal axon outgrowth. In addition to its role in guiding axon pathfinding during neuronal development, SEMA7A has diverse functions in morphogenesis and immune cell control and regulates T cell responses via the α1β1 integrin receptor.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for APC SEMA7A antibody APC-98324-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
