SEMA3A Polyclonal antibody

SEMA3A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27836-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human and More (2)

应用

WB, IF/ICC, ELISA

coll 1, Hsema I, Hsema III, Sema III, SEMA1, SEMA3A, SEMAD, SEMAIII, SEMAL, Semaphorin 3A, Semaphorin III, SemD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPC-12 cells, SH-SY5Y cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

27836-1-AP targets SEMA3A in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human
文献引用反应性human, rat
免疫原 SEMA3A fusion protein Ag27173 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
别名 coll 1, Hsema I, Hsema III, Sema III, SEMA1, SEMA3A, SEMAD, SEMAIII, SEMAL, Semaphorin 3A, Semaphorin III, SemD
计算分子量 771 aa, 89 kDa
观测分子量 89 and 65 kDa
GenBank蛋白编号BC111416
基因名称 SEMA3A
Gene ID (NCBI) 10371
RRIDAB_2880989
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14563
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEMA3A (Semaphorin 3A), a class-3 semaphorin signaling protein, is involved in cell signaling with PlexinA1 and Neuropilin-1 (NRP1) receptors and it is responsible for recruiting dendritic cells into lymphatics. It's widely expressed in different tissues (PMID:10766232). SEMA3A has been shown to exert osteoprotective effects, characterized by increasing bone formation while inhibiting the activation of osteoclast precursor cells (PMID:36598593). However, SEMA3A also modulates the immune system. SEMA3A expression in a myocardial infarct area enhances macrophage polarization and inhibits monocyte migration into the lesion area (PMID:28540528).

实验方案

Product Specific Protocols
WB protocol for SEMA3A antibody 27836-1-APDownload protocol
IF protocol for SEMA3A antibody 27836-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Periodontal Res

Semaphorin 3A attenuates the hypoxia suppression of osteogenesis in periodontal ligament stem cells.

Authors - Xiaochi Chang
humanWB

J Orthop Surg Res

Exosomes promote hFOB1.19 proliferation and differentiation via LINC00520

Authors - Jin Wu
ratWB

Neurosci Lett

TsMS combined with EA promotes functional recovery and axonal regeneration via mediating the miR-539-5p/Sema3A/PlexinA1 signalling axis in sciatic nerve-injured rats

Authors - Xianbin Wang
humanWB

Cell Biochem Biophys

Sema3A Modified PDLSCs Exhibited Enhanced Osteogenic Capabilities and Stimulated Differentiation of Pre-Osteoblasts

Authors - Wen Wang
Loading...