验证数据展示
经过测试的应用
| Positive WB detected in | PC-12 cells, SH-SY5Y cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27836-1-AP targets SEMA3A in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag27173 Product name: Recombinant human SEMA3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 140-235 aa of BC111416 Sequence: ICTYIEIGHHPEDNIFKLENSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHPIRTEQHDSRWLNDPKFISAHLISES 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A |
| 别名 | coll 1, Hsema I, Hsema III, Sema III, SEMA1, SEMA3A, SEMAD, SEMAIII, SEMAL, Semaphorin 3A, Semaphorin III, SemD |
| 计算分子量 | 771 aa, 89 kDa |
| 观测分子量 | 89 and 65 kDa |
| GenBank蛋白编号 | BC111416 |
| 基因名称 | SEMA3A |
| Gene ID (NCBI) | 10371 |
| RRID | AB_2880989 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14563 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SEMA3A (Semaphorin 3A), a class-3 semaphorin signaling protein, is involved in cell signaling with PlexinA1 and Neuropilin-1 (NRP1) receptors and it is responsible for recruiting dendritic cells into lymphatics. It's widely expressed in different tissues (PMID:10766232). SEMA3A has been shown to exert osteoprotective effects, characterized by increasing bone formation while inhibiting the activation of osteoclast precursor cells (PMID:36598593). However, SEMA3A also modulates the immune system. SEMA3A expression in a myocardial infarct area enhances macrophage polarization and inhibits monocyte migration into the lesion area (PMID:28540528).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SEMA3A antibody 27836-1-AP | Download protocol |
| WB protocol for SEMA3A antibody 27836-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Periodontal Res Semaphorin 3A attenuates the hypoxia suppression of osteogenesis in periodontal ligament stem cells. | ||
J Orthop Surg Res Exosomes promote hFOB1.19 proliferation and differentiation via LINC00520 | ||
Neurosci Lett TsMS combined with EA promotes functional recovery and axonal regeneration via mediating the miR-539-5p/Sema3A/PlexinA1 signalling axis in sciatic nerve-injured rats | ||
Sci Adv Selective promotion of sensory innervation-mediated immunoregulation for tissue repair | ||
Cell Biochem Biophys Sema3A Modified PDLSCs Exhibited Enhanced Osteogenic Capabilities and Stimulated Differentiation of Pre-Osteoblasts |
