验证数据展示
经过测试的应用
| Positive WB detected in | LoVo cells, SW480 cells, SKOV-3 cells, SK-BR-3 cells, NIH/3T3 cells, C6 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87925-1-RR targets SEC62 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag29932 Product name: Recombinant human SEC62 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-183 aa of NM_003262 Sequence: MAERRRHKKRIQEVGEPSKEEKAVAKYLRFNCPTKSTNMMGHRVDYFIASKAVDCLLDSKWAKAKKGEEALFTTRESVVDYCNRLLKKQFFHRALKVMKMKYDKDIKKEKDKGKAESGKEEDKKSKKENIKDEKTKKEKEKKKDGEKEESKKEETPGTPKKKETKKKFKLEPHDDQVFLDGNE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | SEC62 homolog (S. cerevisiae) |
| 别名 | Translocation protein SEC62, hTP-1, SEC 62, TP-1, Translocation protein 1 |
| 计算分子量 | 46 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | NM_003262 |
| 基因名称 | SEC62 |
| Gene ID (NCBI) | 7095 |
| RRID | AB_3745830 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q99442 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SEC62 is an integral endoplasmic reticulum (ER) membrane protein. SEC62 and SEC63 form a dimeric complex and play a central role in the translocation of nascent and newly synthesized precursor polypeptides into the ER. SEC62 is associated with poor prognosis in a variety of tumors, such as prostate cancer, hepatocellular cancer, breast cancer, melanoma, and colorectal cancer (PMID: 36262254).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SEC62 antibody 87925-1-RR | Download protocol |
| WB protocol for SEC62 antibody 87925-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
