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验证数据展示

  • WB analysis using 28693-1-AP (same clone as 28693-1-PBS)

    WB analysis using 28693-1-AP (same clone as 28693-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28693-1-AP (SEC62 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28693-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 28693-1-AP (same clone as 28693-1-PBS)

    IHC staining of human ovary tumor using 28693-1-AP (same clone as 28693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28693-1-AP (SEC62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28693-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 28693-1-AP (same clone as 28693-1-PBS)

    IHC staining of human ovary tumor using 28693-1-AP (same clone as 28693-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28693-1-AP (SEC62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28693-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 28693-1-AP (same clone as 28693-1-PBS)

    IF Staining of HepG2 using 28693-1-AP (same clone as 28693-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC62 antibody (28693-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 28693-1-PBS in a different storage buffer formulation.

SEC62 Polyclonal antibody, PBS Only

SEC62 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28693-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Translocation protein SEC62, hTP-1, SEC 62, TP-1, Translocation protein 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28693-1-PBS targets SEC62 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag29932

Product name: Recombinant human SEC62 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-183 aa of NM_003262

Sequence: MAERRRHKKRIQEVGEPSKEEKAVAKYLRFNCPTKSTNMMGHRVDYFIASKAVDCLLDSKWAKAKKGEEALFTTRESVVDYCNRLLKKQFFHRALKVMKMKYDKDIKKEKDKGKAESGKEEDKKSKKENIKDEKTKKEKEKKKDGEKEESKKEETPGTPKKKETKKKFKLEPHDDQVFLDGNE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SEC62 homolog (S. cerevisiae)
别名 Translocation protein SEC62, hTP-1, SEC 62, TP-1, Translocation protein 1
计算分子量 46 kDa
观测分子量52 kDa
GenBank蛋白编号NM_003262
基因名称 SEC62
Gene ID (NCBI) 7095
RRIDAB_3086078
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99442
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SEC62 is a integral endoplasmic reticulum (ER) membrane protein. SEC62 and SEC63 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. SEC62 is associated with poor prognosis in a variety of tumors, such as prostate cancer, hepatocellular cancer, breast cancer , melanoma, colorectal cancer (PMID: 36262254).

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