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验证数据展示

  • WB analysis using 28693-1-AP

    WB analysis using 28693-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28693-1-AP (SEC62 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 28693-1-AP

    IF Staining of HepG2 using 28693-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC62 antibody (28693-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

SEC62 Polyclonal antibody

SEC62 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28693-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Translocation protein SEC62, Translocation protein 1, TP-1, SEC 62, hTP-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells, SW480 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28693-1-AP targets SEC62 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 SEC62 fusion protein Ag29932 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SEC62 homolog (S. cerevisiae)
别名 Translocation protein SEC62, Translocation protein 1, TP-1, SEC 62, hTP-1
计算分子量 46 kDa
观测分子量52 kDa
GenBank蛋白编号NM_003262
基因名称 SEC62
Gene ID (NCBI) 7095
RRIDAB_3086078
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99442
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEC62 is a integral endoplasmic reticulum (ER) membrane protein. SEC62 and SEC63 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. SEC62 is associated with poor prognosis in a variety of tumors, such as prostate cancer, hepatocellular cancer, breast cancer , melanoma, colorectal cancer (PMID: 36262254).

实验方案

Product Specific Protocols
WB protocol for SEC62 antibody 28693-1-APDownload protocol
IF protocol for SEC62 antibody 28693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

ER-phagy restrains inflammatory responses through its receptor UBAC2

Authors - Xing He
View Article