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验证数据展示

  • WB analysis using 20892-1-AP (same clone as 20892-1-PBS)

    WB analysis using 20892-1-AP (same clone as 20892-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 20892-1-AP (same clone as 20892-1-PBS)

    IP experiment of HeLa using 20892-1-AP (same clone as 20892-1-PBS)

    IP result of anti-SEC23IP (IP:20892-1-AP, 4ug; Detection:20892-1-AP 1:1000) with HeLa cells lysate 3600ug. This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 20892-1-AP (same clone as 20892-1-PBS)

    IF Staining of HeLa using 20892-1-AP (same clone as 20892-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC23IP antibody (20892-1-AP) at dilution of 1:0 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 20892-1-AP (same clone as 20892-1-PBS)

    IHC staining of mouse brain using 20892-1-AP (same clone as 20892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20892-1-AP (SEC23IP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 20892-1-AP (same clone as 20892-1-PBS)

    IHC staining of mouse brain using 20892-1-AP (same clone as 20892-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20892-1-AP (SEC23IP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20892-1-PBS in a different storage buffer formulation.

SEC23IP Polyclonal antibody, PBS Only

SEC23IP Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20892-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

MSTP053, p125, SEC23-interacting protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20892-1-PBS targets SEC23IP in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14916

Product name: Recombinant human SEC23IP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 651-1000 aa of BC063800

Sequence: ETLEALSLSEYFSTFEKEKIDMESLLMCTVDDLKEMGIPLGPRKKIANFVEHKAAKLKKAASEKKAVAATSTKGQEQSAQKTKDMASLPSESNEPKRKLPVGACVSSVCVNYESFEVGAGQVSVAYNSLDFEPEIFFALGSPIAMFLTIRGVDRIDENYSLPTCKGFFNIYHPLDPVAYRLEPMIVPDLDLKAVLIPHHKGRKRLHLELKESLSRMGSDLKQGFISSLKSAWQTLNEFARAHTSSTQLQEELEKVANQIKEEEEKQVVEAEKVVESPDFSKDEDYLGKVGMLNGGRRIDYVLQEKPIESFNEYLFALQSHLCYWESEDTALLLLKEIYRTMNISPEQPQH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SEC23 interacting protein
别名 MSTP053, p125, SEC23-interacting protein
计算分子量 1000 aa, 111 kDa
观测分子量 125 kDa
GenBank蛋白编号BC063800
基因名称 SEC23IP
Gene ID (NCBI) 11196
RRIDAB_10896458
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6Y8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SEC23-interacting protein (SEC23IP), also known as p125, is a member of the phosphatidic acid preferring-phospholipase A1 family. SEC23IP interacts with Sec23, a coat component of COPII vesicles which are involved in protein export from the endoplasmic reticulum (ER). SEC23IP localizes to ER exit sites and participates in the organization of this compartment (PMID: 15623529). It may have a role in the early stage of the secretory pathway (PMID: 10400679).

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