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验证数据展示

  • WB analysis using 20892-1-AP

    WB analysis using 20892-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20892-1-AP (SEC23IP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 20892-1-AP

    IHC staining of human heart using 20892-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 20892-1-AP

    IHC staining of human heart using 20892-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20892-1-AP (SEC23IP antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of HeLa using 20892-1-AP

    IP experiment of HeLa using 20892-1-AP

    IP result of anti-SEC23IP (IP:20892-1-AP, 4ug; Detection:20892-1-AP 1:1000) with HeLa cells lysate 3600ug.

  • IF Staining of HeLa using 20892-1-AP

    IF Staining of HeLa using 20892-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC23IP antibody (20892-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SEC23IP Polyclonal antibody

SEC23IP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20892-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

SEC23-interacting protein, p125, MSTP053

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue, HeLa cells, U-251 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20892-1-AP targets SEC23IP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 SEC23IP fusion protein Ag14916 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SEC23 interacting protein
别名 SEC23-interacting protein, p125, MSTP053
计算分子量 1000 aa, 111 kDa
观测分子量 125 kDa
GenBank蛋白编号BC063800
基因名称 SEC23IP
Gene ID (NCBI) 11196
RRIDAB_10896458
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6Y8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEC23-interacting protein (SEC23IP), also known as p125, is a member of the phosphatidic acid preferring-phospholipase A1 family. SEC23IP interacts with Sec23, a coat component of COPII vesicles which are involved in protein export from the endoplasmic reticulum (ER). SEC23IP localizes to ER exit sites and participates in the organization of this compartment (PMID: 15623529). It may have a role in the early stage of the secretory pathway (PMID: 10400679).

实验方案

Product Specific Protocols
WB protocol for SEC23IP antibody 20892-1-APDownload protocol
IHC protocol for SEC23IP antibody 20892-1-APDownload protocol
IF protocol for SEC23IP antibody 20892-1-APDownload protocol
IP protocol for SEC23IP antibody 20892-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
View Article
humanIF

J Cell Biol

Small GTPase ActIvitY ANalyzing (SAIYAN) system: A method to detect GTPase activation in living cells

Authors - Miharu Maeda
View Article