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验证数据展示

  • WB analysis using 68493-1-Ig (same clone as 68493-1-PBS)

    WB analysis using 68493-1-Ig (same clone as 68493-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68493-1-Ig (SEC16A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68493-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 68493-1-Ig (same clone as 68493-1-PBS)

    IF Staining of A431 using 68493-1-Ig (same clone as 68493-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SEC16A antibody (68493-1-Ig, Clone: 1D9A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68493-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68493-1-Ig (same clone as 68493-1-PBS)

    IF Staining of HeLa using 68493-1-Ig (same clone as 68493-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC16A antibody (68493-1-Ig, Clone: 1D9A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68493-1-PBS in a different storage buffer formulation.

SEC16A Monoclonal antibody, PBS Only

SEC16A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68493-1-PBS

产品说明书

CloneNo. 1D9A5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

KIAA0310, p250, RP11 413M3.10, SEC16, SEC16 homolog A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SEC16A Ready-To-Use IHC Kit. SEC16A primary antibody included.

产品信息

68493-1-PBS targets SEC16A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SEC16 homolog A (S. cerevisiae)
别名 KIAA0310, p250, RP11 413M3.10, SEC16, SEC16 homolog A
计算分子量 234 kDa
观测分子量 250-300 kDa
GenBank蛋白编号NM_014866
基因名称 SEC16A
Gene ID (NCBI) 9919
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO15027
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SEC16A, also named as KIAA0310, SEC16 and SEC16L, is required for secretory cargo trafic from the endoplasmic reticulum to the Golgi apparatus. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. SEC16A is required for normal transitional endoplasmic reticulum (tER) organization.

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