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验证数据展示

  • WB analysis using 68493-1-Ig

    WB analysis using 68493-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68493-1-Ig (SEC16A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

SEC16A Monoclonal antibody

SEC16A Monoclonal Antibody for WB, ELISA
Cat No. 68493-1-Ig

产品说明书

CloneNo. 1D9A5

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

FLJ26737, KIAA0310, p250, RP11 413M3.10, SEC16, SEC16 homolog A, SEC16A, SEC16L

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy SEC16A Ready-To-Use IHC Kit. SEC16A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68493-1-Ig targets SEC16A in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SEC16 homolog A (S. cerevisiae)
别名 FLJ26737, KIAA0310, p250, RP11 413M3.10, SEC16, SEC16 homolog A, SEC16A, SEC16L
计算分子量 234 kDa
观测分子量 250-300 kDa
GenBank蛋白编号NM_014866
基因名称 SEC16A
Gene ID (NCBI) 9919
RRIDAB_3085204
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO15027
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEC16A, also named as KIAA0310, SEC16 and SEC16L, is required for secretory cargo trafic from the endoplasmic reticulum to the Golgi apparatus. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. SEC16A is required for normal transitional endoplasmic reticulum (tER) organization.

实验方案

Product Specific Protocols
WB protocol for SEC16A antibody 68493-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols