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SEC14L1 Polyclonal antibody

SEC14L1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 25541-1-AP

Print datasheet

Synonyms

PRELID4A, SEC14 like 1 (S. cerevisiae), SEC14 like protein 1, SEC14L, SEC14L1

验证数据展示

  • WB analysis of Y79 using 25541-1-AP

    WB analysis of Y79 using 25541-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 25541-1-AP (SEC14L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 25541-1-AP

    IP experiment of HEK-293 using 25541-1-AP

    IP result of anti-SEC14L1 (IP:25541-1-AP, 4ug; Detection:25541-1-AP 1:500) with HEK-293 cells lysate 1480 ug.

经过测试的应用

Positive WB detected inHEK-293 cells, Y79 cells, HeLa cells
Positive IP detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25541-1-AP targets SEC14L1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications IP, WB, ELISA
Tested Reactivity human
Immunogen SEC14L1 fusion protein Ag22233 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SEC14-like 1 (S. cerevisiae)
Synonyms PRELID4A, SEC14 like 1 (S. cerevisiae), SEC14 like protein 1, SEC14L, SEC14L1
Calculated Molecular Weight 719 aa, 82 kDa
Observed Molecular Weight 81 kDa, 77 kDa
GenBank Accession NumberBC142979
Gene Symbol SEC14L1
Gene ID (NCBI) 6397
RRIDAB_2880125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92503
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEC14L1 (SEC14-like protein 1) is a member of SEC14 family in mammals with the potential to control local phospholipid content in membranes. SEC14L1 may act as a phospholipid transfer protein. It has also been reported that SEC14L1 functions as a novel negative regulator of RIG-I-mediated antiviral signaling and is involved in innate immunity (PMID: 17092608; 23843640). SEC14L1 has three isoforms with the molecular mass of 81.2kDa, 81.8kDa and 77.1kDa.

实验方案

Product Specific Protocols
WB protocol for SEC14L1 antibody 25541-1-APDownload protocol
IP protocol for SEC14L1 antibody 25541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols