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验证数据展示

  • IP experiment of THP-1 using 30663-1-AP (same clone as 30663-1-PBS)

    IP experiment of THP-1 using 30663-1-AP (same clone as 30663-1-PBS)

    IP result of anti-SDHAF4 (IP:30663-1-AP, 4ug; Detection:30663-1-AP 1:1000) with THP-1 cells lysate 1800 ug. This data was developed using the same antibody clone with 30663-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 30663-1-AP (same clone as 30663-1-PBS)

    IHC staining of human breast cancer using 30663-1-AP (same clone as 30663-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 30663-1-AP (C6orf57 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30663-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 30663-1-AP (same clone as 30663-1-PBS)

    IHC staining of human cervical cancer using 30663-1-AP (same clone as 30663-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 30663-1-AP (C6orf57 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30663-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 30663-1-AP (same clone as 30663-1-PBS)

    IF Staining of A431 using 30663-1-AP (same clone as 30663-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C6orf57 antibody (30663-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30663-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 30663-1-AP (same clone as 30663-1-PBS)

    IF Staining of U2OS using 30663-1-AP (same clone as 30663-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using C6orf57 antibody (30663-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30663-1-PBS in a different storage buffer formulation.

SDHAF4 Polyclonal antibody, PBS Only

SDHAF4 Polyclonal Antibody for IHC, IF/ICC, IP, Indirect ELISA
Cat No. 30663-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, IP, Indirect ELISA

别名

C6orf57, SDH assembly factor 4, Succinate dehydrogenase assembly factor 4, mitochondrial, Succinate Dehydrogenase Complex Assembly Factor 4, UPF0369 protein C6orf57

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30663-1-PBS targets SDHAF4 in IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33549

Product name: Recombinant human C6orf57 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-108 aa of BC104649

Sequence: ARSPLLCHSLRKTSSSQGGKSELVKQSLKKPKLPEGRFDAPEDSHLEKEPLEKFPDDVNPVTKEKGGPRGPEPTRYGDWERKGRCIDF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 6 open reading frame 57
别名 C6orf57, SDH assembly factor 4, Succinate dehydrogenase assembly factor 4, mitochondrial, Succinate Dehydrogenase Complex Assembly Factor 4, UPF0369 protein C6orf57
观测分子量15 kDa
GenBank蛋白编号BC104649
基因名称 C6orf57
Gene ID (NCBI) 135154
RRIDAB_3742363
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5VUM1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SDHAF4 (Succinate Dehydrogenase Assembly Factor 4) is a key mitochondrial protein specifically responsible for assisting in the assembly and stability of mitochondrial complex II (Succinate Dehydrogenase, SDH). This complex plays a central role in cellular energy metabolism by participating simultaneously in the tricarboxylic acid (TCA) cycle and the electron transport chain. Its function involves binding to the SDHA (flavoprotein) subunit, which is already associated with FAD, thereby preventing excessive ROS generation. It further promotes the assembly of SDHA with the iron-sulfur protein SDHB to form the catalytic dimer. This ensures the proper integration of complex II into the inner mitochondrial membrane, allowing it to participate in both the TCA cycle and electron transport. Deficiency of SDHAF4 leads to reduced SDH activity, accumulation of succinate, and elevated mitochondrial superoxide levels, which are associated with certain cardiomyopathies and tumor metabolic reprogramming.

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