验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human liver tissue, mouse heart tissue |
| Positive IHC detected in | human breast cancer tissue, human gliomas tissue, rat colon tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10407-1-AP targets Syntenin 2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag0668 Product name: Recombinant human SDCBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-207 aa of BC002727 Sequence: MVAPVTGYSLGVRRAEIKPGVREIHLCKDERGKTGLRLRKVDQGLFVQLVQANTPASLVGLRFGDQLLQIDGRDCAGWSSHKAHQVVKKASGDKIVVVVRDRPFQRTVTMHKDSMGHVGFVIKKGKIVSLVKGSSAARNGLLTNHYVCEVDGQNVIGLKDKKIMEILATAGNVVTLTIIPSVIYEHMVKKLPPVLLHHTMDHSIPDA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | syndecan binding protein (syntenin) 2 |
| 别名 | Syntenin-2, Syndecan-binding protein 2, SITAC18, SITAC, Similar to TACIP18 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 35-37 kDa |
| GenBank蛋白编号 | BC002727 |
| 基因名称 | Syntenin 2 |
| Gene ID (NCBI) | 27111 |
| RRID | AB_2183161 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H190 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Syndecan-binding protein 2 (SDCBP2, Syntenin 2) is a protein containing two PDZ domains. PDZ domains facilitate protein-protein interactions by binding to the cytoplasmic C-terminus of transmembrane proteins. SDCBP2 binds with high affinity to PIP2 and is targeted to the plasma membrane, nucleoli and nuclear speckles via its PDZ domains (PMID: 15961997). SDCBP2 exists in two alternatively spliced variants, 2α and 2β, which differ in the lengths of their N-terminal regions. (PMID: 15276154)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Syntenin 2 antibody 10407-1-AP | Download protocol |
| WB protocol for Syntenin 2 antibody 10407-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Virol Human papillomavirus type 8 E6 oncoprotein inhibits transcription of the PDZ protein syntenin-2. | ||
Front Microbiol HPV8-E6 Interferes with Syntenin-2 Expression through Deregulation of Differentiation, Methylation and Phosphatidylinositide-Kinase Dependent Mechanisms.
| ||
Front Neurosci MiR-409-5p as a Regulator of Neurite Growth Is Down Regulated in APP/PS1 Murine Model of Alzheimer's Disease.
| ||
Gut Pathog Virulence factors and mechanisms of paediatric pneumonia caused by Enterococcus faecalis |
