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验证数据展示

  • WB analysis of U-251 using 83786-4-RR

    WB analysis of U-251 using 83786-4-RR

    U-251 cells were subjected to SDS PAGE followed by western blot with 83786-4-RR (SCPEP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 83786-4-RR

    WB analysis of A431 using 83786-4-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 83786-4-RR (SCPEP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 83786-4-RR

    IHC staining of human stomach cancer using 83786-4-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83786-4-RR (SCPEP1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Affinity and Kinetic Characterization of 83786-4-RR

    Affinity and Kinetic Characterization of 83786-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83786-4-RR against Human SCPEP1 were performed. The affinity constant is below 1 pM.

SCPEP1 Recombinant monoclonal antibody

SCPEP1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83786-4-RR

产品说明书

CloneNo. 240886B6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

RISC, Retinoid-inducible serine carboxypeptidase, MSTP034, HSCP1, EC:3.4.16.-

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU-251 cells, A431 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83786-4-RR targets SCPEP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag31381

Product name: Recombinant human SCPEP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 221-452 aa of BC072405

Sequence: LLEDKGLAEVSKVAEQVLNAVNKGLYREATELWGKAEMIIEQNTDGVNFYNILTKSTPTSTMESSLEFTQSHLVCLCQRHVRHLQRDALSQLMNGPIRKKLKIIPEDQSWGGQATNVFVNMEEDFMKPVISIVDELLEAGINVTVYNGQLDLIVDTMGQEAWVRKLKWPELPKFSQLKWKALYSDPKSLETSAFVKSYKNLAFYWILKAGHMVPSDQGDMALKMMRLVTQQE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 serine carboxypeptidase 1
别名 RISC, Retinoid-inducible serine carboxypeptidase, MSTP034, HSCP1, EC:3.4.16.-
计算分子量 452 aa, 51 kDa
观测分子量33 kDa, 45~51 kDa
GenBank蛋白编号BC072405
基因名称 SCPEP1
Gene ID (NCBI) 59342
RRIDAB_3671378
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9HB40
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCPEP1 encodes retinoid-inducible serine carboxypeptidase (PMID:12975309). SCPEP1 and Cathepsin A play roles in regulation of vasoconstriction via inactivation of endothelin-1 (PubMed:24586188). The protein is widely expressed during mouse embryogenesis by immunohistochemical (PubMed:16461364). Scpep1 is proteolytically cleaved to a mature enzyme which is smaller than 51 kDa (PMID:16461364).

实验方案

Product Specific Protocols
IHC protocol for SCPEP1 antibody 83786-4-RRDownload protocol
WB protocol for SCPEP1 antibody 83786-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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