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验证数据展示

  • WB analysis using 29781-1-AP (same clone as 29781-1-PBS)

    WB analysis using 29781-1-AP (same clone as 29781-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 29781-1-AP (SCPEP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29781-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse embryo using 29781-1-AP (same clone as 29781-1-PBS)

    IHC staining of mouse embryo using 29781-1-AP (same clone as 29781-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29781-1-AP (SCPEP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29781-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse embryo using 29781-1-AP (same clone as 29781-1-PBS)

    IHC staining of mouse embryo using 29781-1-AP (same clone as 29781-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29781-1-AP (SCPEP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29781-1-PBS in a different storage buffer formulation.

SCPEP1 Polyclonal antibody, PBS Only

SCPEP1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29781-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

RISC, EC:3.4.16.-, HSCP1, MSTP034, Retinoid-inducible serine carboxypeptidase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29781-1-PBS targets SCPEP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag31396

Product name: Recombinant human SCPEP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 221-452 aa of BC072405

Sequence: LLEDKGLAEVSKVAEQVLNAVNKGLYREATELWGKAEMIIEQNTDGVNFYNILTKSTPTSTMESSLEFTQSHLVCLCQRHVRHLQRDALSQLMNGPIRKKLKIIPEDQSWGGQATNVFVNMEEDFMKPVISIVDELLEAGINVTVYNGQLDLIVDTMGQEAWVRKLKWPELPKFSQLKWKALYSDPKSLETSAFVKSYKNLAFYWILKAGHMVPSDQGDMALKMMRLVTQQE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serine carboxypeptidase 1
别名 RISC, EC:3.4.16.-, HSCP1, MSTP034, Retinoid-inducible serine carboxypeptidase
计算分子量 452 aa, 51 kDa
观测分子量30 kDa, 51 kDa
GenBank蛋白编号BC072405
基因名称 SCPEP1
Gene ID (NCBI) 59342
RRIDAB_2923608
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HB40
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SCPEP1 is synthesized as a ~51-55 kDa precursor (zymogen) containing a signal peptide and five N-glycosylation sites. During lysosomal maturation, it undergoes proteolytic cleavage and N-glycan trimming, producing multiple forms that can appear at ~30 kDa on WB. (PMID: 19187242, 16461364)

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