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验证数据展示

  • WB analysis using 29781-1-AP

    WB analysis using 29781-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 29781-1-AP (SCPEP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse embryo using 29781-1-AP

    IHC staining of mouse embryo using 29781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29781-1-AP (SCPEP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse embryo using 29781-1-AP

    IHC staining of mouse embryo using 29781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29781-1-AP (SCPEP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SCPEP1 Polyclonal antibody

SCPEP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29781-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

RISC, EC:3.4.16.-, HSCP1, MSTP034, Retinoid-inducible serine carboxypeptidase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29781-1-AP targets SCPEP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原

CatNo: Ag31396

Product name: Recombinant human SCPEP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 221-452 aa of BC072405

Sequence: LLEDKGLAEVSKVAEQVLNAVNKGLYREATELWGKAEMIIEQNTDGVNFYNILTKSTPTSTMESSLEFTQSHLVCLCQRHVRHLQRDALSQLMNGPIRKKLKIIPEDQSWGGQATNVFVNMEEDFMKPVISIVDELLEAGINVTVYNGQLDLIVDTMGQEAWVRKLKWPELPKFSQLKWKALYSDPKSLETSAFVKSYKNLAFYWILKAGHMVPSDQGDMALKMMRLVTQQE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serine carboxypeptidase 1
别名 RISC, EC:3.4.16.-, HSCP1, MSTP034, Retinoid-inducible serine carboxypeptidase
计算分子量 452 aa, 51 kDa
观测分子量30 kDa, 51 kDa
GenBank蛋白编号BC072405
基因名称 SCPEP1
Gene ID (NCBI) 59342
RRIDAB_2923608
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HB40
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCPEP1 is synthesized as a ~51-55 kDa precursor (zymogen) containing a signal peptide and five N-glycosylation sites. During lysosomal maturation, it undergoes proteolytic cleavage and N-glycan trimming, producing multiple forms that can appear at ~30 kDa on WB. (PMID: 19187242, 16461364)

实验方案

Product Specific Protocols
IHC protocol for SCPEP1 antibody 29781-1-APDownload protocol
WB protocol for SCPEP1 antibody 29781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Immunol

SCPEP1(+) basal cells are associated with the remodeling of oxidative stress signaling networks in idiopathic pulmonary fibrosis.

Authors - Xiang Zhou
View Article
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