验证数据展示
发表文章中的应用
| WB | See 4 publications below |
产品信息
19182-1-AP targets SCP2/SCPx in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | rat |
| 免疫原 | SCP2/SCPx fusion protein Ag6002 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sterol carrier protein 2 |
| 别名 | NLTP, NSL TP, SCP 2, SCP chi, SCP X, SCP2, sterol carrier protein 2, Sterol carrier protein X |
| 计算分子量 | 59 kDa |
| 观测分子量 | 46 kDa, 58 kDa |
| GenBank蛋白编号 | BC067108 |
| 基因名称 | SCP2 |
| Gene ID (NCBI) | 6342 |
| RRID | AB_10603471 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P22307 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. 19182-1-AP antibody recognizes the intact 58 kDa SCPx protein as well as 44-46 kDa thiolase.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SCP2/SCPx antibody 19182-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem A Cargo-centered Perspective on the PEX5 Receptor-mediated Peroxisomal Protein Import Pathway. | ||
FEBS J Membrane topologies of PEX13 and PEX14 provide new insights on the mechanism of protein import into peroxisomes. | ||
J Biol Chem The peroxisomal matrix protein translocon is a large cavity-forming protein assembly into which PEX5 protein enters to release its cargo. | ||
Methods Mol Biol Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays. |
