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验证数据展示

  • WB analysis using 83027-4-RR (same clone as 83027-4-PBS)

    WB analysis using 83027-4-RR (same clone as 83027-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83027-4-RR (SCN4B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83027-4-PBS in a different storage buffer formulation.

  • IHC staining of rat dorsal root ganglion using 83027-4-RR (same clone as 83027-4-PBS)

    IHC staining of rat dorsal root ganglion using 83027-4-RR (same clone as 83027-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 83027-4-RR (SCN4B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83027-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83027-4-RR (same clone as 83027-4-PBS)

    FC experiment of HeLa using 83027-4-RR (same clone as 83027-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human SCN4B (83027-4-RR, Clone:230297B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83027-4-PBS in a different storage buffer formulation.

SCN4B Recombinant antibody, PBS Only

SCN4B Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 83027-4-PBS

产品说明书

CloneNo. 230297B11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

Sodium channel subunit beta-4, Sodium channel subunit beta 4, 230297B11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83027-4-PBS targets SCN4B in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SCN4B fusion protein Ag34123 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sodium channel, voltage-gated, type IV, beta
别名 Sodium channel subunit beta-4, Sodium channel subunit beta 4, 230297B11
计算分子量25 kDa
观测分子量 37 kDa
GenBank蛋白编号NM_174934.3
基因名称 SCN4B
Gene ID (NCBI) 6330
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IWT1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mammalian sodium channels are heterotrimers, composed of a central, pore-forming alpha subunit and two auxiliary beta subunits (PMID: 11486343). SCN4B (sodium channel subunit beta-4) is one of several sodium channel beta subunits, which modulate channel gating kinetics. SCN4B has an N-terminal signal peptide, a large extracellular domain predicted to form an Ig-like fold, a single transmembrane alpha helix, and a short cytoplasmic C terminus. Defects in SCN4B gene are a cause of long QT syndrome type 10 (LQT10).