- Featured Product
- KD/KO Validated
SCIN Polyclonal antibody
SCIN Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11579-1-AP targets SCIN in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | SCIN fusion protein Ag2168 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | scinderin |
| Synonyms | Adseverin, KIAA1905, SCIN, scinderin |
| Calculated Molecular Weight | 715 aa, 81 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC021090 |
| Gene Symbol | SCIN |
| Gene ID (NCBI) | 85477 |
| RRID | AB_10641047 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6U3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SCIN antibody 11579-1-AP | Download protocol |
| IHC protocol for SCIN antibody 11579-1-AP | Download protocol |
| IF protocol for SCIN antibody 11579-1-AP | Download protocol |
| IP protocol for SCIN antibody 11579-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
FEBS Open Bio Loss of scinderin decreased expression of epidermal growth factor receptor and promoted apoptosis of castration-resistant prostate cancer cells.
| ||
World J Clin Oncol Scinderin promotes glioma cell migration and invasion via remodeling actin cytoskeleton
| ||
Cell Biosci Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos | ||
Acta Neuropathol Commun Synaptic proteomics reveal distinct molecular signatures of cognitive change and C9ORF72 repeat expansion in the human ALS cortex |