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验证数据展示

  • WB analysis using 13375-1-AP

    WB analysis using 13375-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13375-1-AP (SCFD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 13375-1-AP

    IHC staining of human colon cancer using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 13375-1-AP

    IHC staining of human colon cancer using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 Antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of COLO 320 using 13375-1-AP

    IP experiment of COLO 320 using 13375-1-AP

    IP result of anti-SCFD2 (IP:13375-1-AP, 4ug; Detection:13375-1-AP 1:600) with COLO 320 cells lysate 1680 ug.

  • IHC staining of human colon using 13375-1-AP

    IHC staining of human colon using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13375-1-AP (SCFD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 13375-1-AP

    IHC staining of human colon cancer using 13375-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13375-1-AP (SCFD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SCFD2 Polyclonal antibody

SCFD2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13375-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

Sec1 family domain-containing protein 2, STXBP1L1, Syntaxin-binding protein 1-like 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13375-1-AP targets SCFD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4197

Product name: Recombinant human SCFD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC032453

Sequence: MSASGVLSFTQQGWEQVLAKVKRAVVYLDAACAESLHWGCGSTRLLEAVGGPDCHLREFEPDAIGGGAKQPKAVFVLSCLLKGRTVEILRDIICRSHFQYCVVVTTVSHAVHLTANHVPAAAAAEMEGQQPVFEQLEEKLCEWMGNMNYTAEVFHVPLLLAPVAPHFALTPAFASLFPLLPQDVHLLNSARPDKRKLGSLGDVDSTTLTPELLLQIRCLVSGLSSLCEHLGVREECFAVGSLSQVIAADLANYAPAKNRKKTAAGRASVVFVDRTLDLTGAVGHHGDNLVEKIISALPQLPGHTNDVMVNMIALTALHTEEENYNVVAPGCLSQSSDTTAKALWEALLNTK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sec1 family domain containing 2
别名 Sec1 family domain-containing protein 2, STXBP1L1, Syntaxin-binding protein 1-like 1
计算分子量 684 aa, 75 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC032453
基因名称 SCFD2
Gene ID (NCBI) 152579
RRIDAB_10646473
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WU76
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SCFD2 antibody 13375-1-APDownload protocol
IHC protocol for SCFD2 antibody 13375-1-APDownload protocol
IP protocol for SCFD2 antibody 13375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols