验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67200 targets SBDS in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag11731 Product name: Recombinant human SBDS protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-250 aa of BC065700 Sequence: MSIFTPTNQIRLTNVAVVRMKRAGKRFEIACYKNKVVGWRSGVEKDLDEVLQTHSVFVNVSKGQVAKKEDLISAFGTDDQTEICKQILTKGEVQVSDKERHTQLEQMFRDIATIVADKCVNPETKRPYTVILIERAMKDIHYSVKTNKSTKQQALEVIKQLKEKMKIERAHMRLRFILPVNEGKKLKEKLKPLIKVIESEDYGQQLEIVCLIDPGCFREIDELIKKETKGKGSLEVLNLKDVEEGDEKFE 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Shwachman-Bodian-Diamond syndrome |
| 别名 | 1F8A10, CGI 97, CGI-97, SDS, Shwachman-Bodian-Diamond syndrome protein |
| 计算分子量 | 250 aa, 29 kDa |
| 观测分子量 | 29-31 kDa |
| GenBank蛋白编号 | BC065700 |
| 基因名称 | SBDS |
| Gene ID (NCBI) | 51119 |
| RRID | AB_3673516 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9Y3A5 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Shwachman-Bodian-Diamond syndrome (SBDS) is a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The protein may function in RNA metabolism. Mutations within its gene are associated with Shwachman-Bodian-Diamond syndrome.This gene encodes a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The encoded protein may function in RNA metabolism. Mutations within this gene are associated with Shwachman-Bodian-Diamond syndrome. An alternative transcript has been described, but its biological nature has not been determined. This gene has a closely linked pseudogene that is distally located.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 SBDS antibody CL594-67200 | Download protocol |
| WB protocol for CL594 SBDS antibody CL594-67200 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
